Genetic diversity and antimicrobial susceptibility of Campylobacter jejuni isolates associated with sheep abortion in the United States and Great Britain.
Genetic diversity and antimicrobial susceptibility of Campylobacter jejuni isolates associated with sheep abortion in the United States and Great Britain.
复制标题
与美国和英国绵羊流产相关的空肠弯曲杆菌分离株的遗传多样性和抗菌药物敏感性。
DOI：
10.1128/jcm.00355-14
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发表时间：
2014
影响因子：
9.4
通讯作者：
Zhang,Qijing
中科院分区：
文献类型：
--
作者：
Wu,Zuowei;Sippy,Rachel;Sahin,Orhan;Plummer,Paul;Vidal,Ana;Newell,Diane;Zhang,Qijing
弯曲杆菌感染是全世界绵羊流产的主要原因。从历史上看，遗传多样性的胎儿弯曲杆菌和空肠弯曲杆菌菌株与此类感染有关，但自 2003 年以来，一种高致病性、耐四环素的空肠弯曲杆菌克隆（名为 SA）已成为美国绵羊流产的主要原因。 SA克隆是否存在于美国早期的流产菌株中（2000年之前），以及是否与美国以外的绵羊流产有关，目前尚不清楚。在这里，我们分析了不同时期从美国绵羊流产中收集的 54 个空肠弯曲菌分离株，并使用多位点序列分型 (MLST)、脉冲场凝胶电泳 (PFGE) 和基于阵列的比较基因组杂交 (CGH)，将它们与 2002 年至 2008 年英国绵羊流产相关的 42 个空肠弯曲菌分离株进行了比较。尽管克隆 SA (ST-8) 存在于美国早期分离株中，但它并不像美国晚期分离株那样具有四环素抗性（19% 对 100%）或占主导地位（66% 对 91%）。相比之下，来自英国的空肠弯曲菌分离株具有遗传多样性，包含 19 个 ST，但缺乏 ST-8。代表性菌株的PFGE和CGH分析进一步证实了流产分离株的群体结构。值得注意的是，英国的分离株基本上对大多数经过测试的抗生素（包括四环素）敏感，而美国晚期的分离株普遍对这种抗生素具有耐药性，这可以用四环素在美国而非英国普遍用于控制绵羊流产来解释。这些结果表明，克隆SA在绵羊流产中占主导地位是美国独有的，四环素的使用可能促进了对这种高致病性克隆的选择。
Campylobacter infection is a leading cause of ovine abortion worldwide. Historically, genetically diverse Campylobacter fetus and Campylobacter jejuni strains have been implicated in such infections, but since 2003 a highly pathogenic, tetracycline-resistant C. jejuni clone (named SA) has become the predominant cause of sheep abortions in the United States. Whether clone SA was present in earlier U.S. abortion isolates (before 2000) and is associated with sheep abortions outside the United States are unknown. Here, we analyzed 54 C. jejuni isolates collected from U.S. sheep abortions at different time periods and compared them with 42 C. jejuni isolates associated with sheep abortion during 2002 to 2008 in Great Britain, using multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and array-based comparative genomic hybridization (CGH). Although clone SA (ST-8) was present in the early U.S. isolates, it was not as tetracycline resistant (19% versus 100%) or predominant (66% versus 91%) as it was in the late U.S isolates. In contrast, C. jejuni isolates from Great Britain were genetically diverse, comprising 19 STs and lacking ST-8. PFGE and CGH analyses of representative strains further confirmed the population structure of the abortion isolates. Notably, the Great Britain isolates were essentially susceptible to most tested antibiotics, including tetracycline, while the late U.S. isolates were universally resistant to this antibiotic, which could be explained by the common use of tetracyclines for control of sheep abortions in the United States but not in Great Britain. These results suggest that the dominance of clone SA in sheep abortions is unique to the United States, and the use of tetracyclines may have facilitated selection of this highly pathogenic clone.