Isoprenoid geranylgeraniol: the influence on cell characteristics of endothelial progenitor cells after bisphosphonate therapy in vitro
Isoprenoid geranylgeraniol: the influence on cell characteristics of endothelial progenitor cells after bisphosphonate therapy in vitro
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类异戊二烯香叶基香叶醇：体外双膦酸盐治疗后对内皮祖细胞细胞特性的影响
DOI：
10.1007/s00784-014-1394-z
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发表时间：
2015
影响因子：
3.4
通讯作者：
Christian Walter
中科院分区：
文献类型：
--
作者：
A. Pabst;Maximilian Krüger;T. Ziebart;C. Jacobs;Christian Walter
ObjectivesGeranylgeraniol (GGOH) has been reported as a potential treatment option for bisphosphonate-associated osteonecrosis of the jaws (BP-ONJ). The aim of this study was to analyze the effects of GGOH on endothelial progenitor cells (EPC) after bisphosphonate treatment in vitro.Materials and methodsEPC were incubated with different nitrogen (N-BPs: ibandronate, pamidronate, zoledronate) and a non-nitrogen-containing bisphosphonates (NN-BP: clodronate) with and without GGOH. Cell viability was measured by MTT and PrestoBlue assay. Migration ability was analyzed with a Boyden and Scratch assay. Apoptosis rates were determined by colony-forming, Tunel and ToxiLight assays.ResultsNegative effects of N-BPs on EPC were shown in all tests without GGOH. The substitution of GGOH demonstrated significantly increased cell viability (MTT: p each N-BP ≤0.004; PrestoBlue: p each N-BP <0.001) and migration ability (Boyden: p each N-BP <0.001; Scratch: p each N-BP <0.001). Concerning the apoptosis rates, increased EPC colony densities (p each N-BP ≤0.009), decreased numbers of apoptotic cells in the Tunel assay (p each N-BP <0.001), and a decreased adenylate kinase release in the ToxiLight assay (p each N-BP ≤0.03) were observed. For the clodronate-treated cells, no significant differences could be detected with or without GGOH in any assay (p each N-BP/NN-BP >0.05).ConclusionsGGOH cell treatment reversed the negative effects of bisphosphonates on EPC.Clinical relevanceThese findings support the hypothesis that systemic or local GGOH treatment might lead to new therapeutic strategies for BP-ONJ.
影响因子：
20.1
作者：
Asahara, T;Masuda, H;Isner, JM
通讯作者：
Isner, JM