NEURAL CORRELATES OF OLFACTORY LEARNING-PARADIGMS IN AN IDENTIFIED NEURON IN THE HONEYBEE BRAIN
NEURAL CORRELATES OF OLFACTORY LEARNING-PARADIGMS IN AN IDENTIFIED NEURON IN THE HONEYBEE BRAIN
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DOI：
10.1152/jn.1993.69.2.609
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发表时间：
1993-02-01
影响因子：
2.5
通讯作者：
MAUELSHAGEN, J
中科院分区：
文献类型：
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作者：
MAUELSHAGEN, J
1.敏感化和经典的气味条件反射的长鼻延伸功能进行了分析，从一个单一的识别神经元（PE 1-神经元）在中央蜜蜂大脑的重复细胞内记录。这种神经元属于从蘑菇体的柄产生的“外在细胞”类，在中脑和侧前脑中有广泛的分支。记录在分离的蜂头上进行。两个不同的系列或生理实验进行了使用类似的时间序列的刺激，在以前的行为实验。在第一个系列中，一组动物用于单一条件试验[条件刺激（CS），康乃馨;非条件刺激（US），触角和吻的蔗糖溶液），第二组用于敏化（敏化刺激，触角和/或吻的蔗糖溶液），第三组用作对照（无蔗糖刺激）。在第二个系列中，应用差异条件反射范式（配对气味CS+，康乃馨;未配对气味CS-，橙子开花）来测试条件反射效应的关联性质。PE 1-神经元在训练程序之前表现出特征性的爆发式气味反应。治疗导致不同的尖峰频率调制的这种反应，这是特定的非联想和联想刺激范例应用。在差分条件反射过程中，有动态向上和向下调制的尖峰频率和DC电位的基础上的CS+的反应。总的来说，只有短暂的变化，在分钟范围内被忽略。致敏程序的结果表明两种性质不同的US途径。敏化和一次试验条件反射之间的比较显示了非联想和联想刺激范例对PE 1神经元的反应行为的差异效应。差分条件反射程序的结果表明，所观察到的效果的一次审判条件反射范式是一个联想的性质，并可能有调制，这是特定的单次和多次审判条件反射程序。据推测，PE 1-神经元是一个可能的元素参与短期收购，而不是在长期存储，在蜜蜂的联想嗅觉记忆。
1. Sensitization and classical odor conditioning of the proboscis extension reflex were functionally analyzed by repeated intracellular recordings from a single identified neuron (PE1-neuron) in the central bee brain. This neuron belongs to the class of ''extrinsic cells'' arising from the pedunculus of the mushroom bodies and has extensive arborizations in the median and lateral protocerebrum. The recordings were performed on isolated bee heads.2. Two different series or physiological experiments were carried out with the use of a similar temporal succession of stimuli as in previous behavioral experiments. In the first series, one group of animals was used for a single conditioning trial [conditioned stimulus (CS), carnation; unconditioned stimulus (US), sucrose solution to the antennae and proboscis), a second group was used for sensitization (sensitizing stimulus, sucrose solution to the antennae and/or proboscis), and the third group served as control (no sucrose stimulation). In the second series, a differential conditioning paradigm (paired odor CS+, carnation; unpaired odor CS-, orange blossom) was applied to test the associative nature of the conditioning effect.3. The PE1-neuron showed a characteristic burstlike odor response before the training procedures. The treatments resulted in different spike-frequency modulations of this response, which were specific for the nonassociative and associative stimulus paradigms applied. During differential conditioning, there are dynamic up and down modulations of spike frequencies and of the DC potentials underlying the responses to the CS+. Overall, only transient changes in the minute range were observed.4. The results of the sensitization procedures suggest two qualitatively different US pathways. The comparison between sensitization and one-trial conditioning shows differential effects of non-associative and associative stimulus paradigms on the response behavior of the PE1-neuron. The results of the differential conditioning procedure reveal that the effect observed for the one-trial conditioning paradigm is of an associative nature and that there might be modulations, which are specific for single and multiple trial conditioning procedures. It is hypothesized that the PE1-neuron is a possible element involved in the short-term acquisition, rather than in the long-term storage, of an associative olfactory memory in the honeybee.