A way to probe the microenvironment of free sulfhydryls in intact proteins with a series of monofunctional organic mercurials.
A way to probe the microenvironment of free sulfhydryls in intact proteins with a series of monofunctional organic mercurials.
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DOI：
10.1002/chem.201200901
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Ming Xu;Limin Yang;Qiuquan Wang
中科院分区：
文献类型：
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作者：
Ming Xu;Limin Yang;Qiuquan Wang
使用不同尺寸和电子性质的单官能有机汞（MFOHgs）来评估完整蛋白质中SH基团的可及性。当用尺寸增加的MFOHgs处理时，蛋白质如BLG和OVA的构象变化被用作这些蛋白质内所含SH基团的可接近性的量度。
Monofunctional organic mercurials (MFOHgs) of different sizes and electronic properties were used to evaluate the accessibility of SH groups in intact proteins. The change in conformation of proteins, such as BLG and OVA, when treated with MFOHgs of increasing size was used as a measure of the accessibility of SH groups contained within these proteins.