EFFECTS OF BORDETELLA-BRONCHISEPTICA DERMONECROTIC TOXIN ON THE STRUCTURE AND FUNCTION OF OSTEOBLASTIC CLONE MC3T3-E1 CELLS
EFFECTS OF BORDETELLA-BRONCHISEPTICA DERMONECROTIC TOXIN ON THE STRUCTURE AND FUNCTION OF OSTEOBLASTIC CLONE MC3T3-E1 CELLS
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DOI：
10.1128/iai.59.3.1112-1116.1991
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发表时间：
1991-03-01
影响因子：
3.1
通讯作者：
KUME, K
中科院分区：
文献类型：
--
作者：
HORIGUCHI, Y;NAKAI, T;KUME, K
研究了支气管败血波氏杆菌皮肤坏死毒素对成骨细胞克隆株MC3T3-E1结构和功能的影响。毒素诱导细胞形态发生变化，由纺锤形变为具有许多气泡的球形。经毒素处理的细胞存活，并生长形成融合细胞层，由不规则形状的细胞和多核细胞组成。该毒素在10pg~10 ng/ml浓度范围内呈剂量依赖性抑制细胞内碱性磷酸酶活力的升高，并可减少I型胶原在细胞内的积聚。由于高碱性磷酸酶活性和胶原蛋白的积累与细胞向成骨细胞的分化密切相关，因此认为支气管败血杆菌皮肤坏死毒素可能会损害细胞的分化能力。
The effects of Bordetella bronchiseptica dermonecrotic toxin on the structure and function of a clonal osteoblastic cell line, MC3T3-E1, were investigated. The toxin induced a morphological change in the cells from a spindle shape to a spherical form with many blebs. The toxin-treated cells were viable and grew to form confluent cell layers composed of irregularly shaped cells and multinuclear cells. The toxin inhibited elevation of alkaline phosphatase activity in the cells in a dose-dependent manner at concentrations from 10 pg to 10 ng/ml. The accumulation of type I collagen in the cells was also reduced by the toxin. Since high alkaline phosphatase activity and accumulation of collagen are closely linked to differentiation of the cells into osteoblasts, it is considered likely that B. bronchiseptica dermonecrotic toxin impairs the ability of the cells to differentiate.