Analysis on Terminal Enzyme and ATPase Activities between CMS Lines and Their Maintainers during Vegetative Period of Sugarbeet
Analysis on Terminal Enzyme and ATPase Activities between CMS Lines and Their Maintainers during Vegetative Period of Sugarbeet
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发表时间：
2008
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通讯作者：
Tian Zi-hua
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作者：
Tian Zi-hua
为了研究甜菜细胞质雄性不育（CMS）与能量代谢的关系，采用212A、2033A和2034A 3个不育系及其维持系212B、2033B和2034B，分析了甜菜营养期4个关键时期叶片细胞色素氧化酶（COD）、过氧化物酶（POD）和过氧化氢酶（CAT） 3种末端酶的活性以及ATP酶（ATP酶）的活性。结果表明，CMS系的COD活性均显著低于保持系，表明CMS系的呼吸强度和能量产生均低于保持系。在营养期，ATPase活性在CMS系和保持系之间差异不大，变化不大，说明二者所需能量相同。CMS系POD和CAT活性的提高可能与H2O2含量的增加有关。研究表明，能量缺乏可能导致甜菜不育系发生营养期。
In order to study the relation between cytoplasmic male sterile(CMS)and energy metobolism in sugarbeet,three CMS lines,212A,2033A and 2034A and their maintainers,212B,2033B and 2034B were used to analyze the activities of three terminal enzyme,cytochrome oxidase(COD),peroxidase(POD)and catalase(CAT),as well as activity of ATP enzyme(ATPase)of leaves in 4 critical phases of sugarbeet vegetative period.The results showed that activities of COD in CMS lines were all dramatically lower than those of their maintainers,suggesting that respiratory intensity and energy produced in CMS lines were lower than those in their maintainers.The activities of ATPase had little differences and changed little between CMS lines and their maintainers during vegetative period,showing that the both needed same amount of energy.The higher activities of POD and CAT in CMS lines might be resulted from their H2O2 content increasing.The study showed that energy deficiency might lead to CMS occurring vegetative period of sugarbeet CMS lines.