VirE1-mediated resistance to crown gall in transgenic Arabidopsis thaliana
VirE1-mediated resistance to crown gall in transgenic Arabidopsis thaliana
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DOI：
10.1094/phyto-96-0105
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发表时间：
2006-01-01
期刊：
影响因子：
3.2
通讯作者：
Ream, W
中科院分区：
文献类型：
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作者：
Humann, J;Andrews, S;Ream, W
尽管目前有生物防治方法，由农杆菌引起的冠瘿病仍然是一个严重的农业问题。农杆菌将单链DNA （t链）与几种毒力蛋白（包括单链DNA结合蛋白（VirE2））一起转移到植物细胞中。在植物细胞中，t链受到核酸酶的保护，并被VirE2靶向到细胞核中，这对于t链的有效传递（转移和整合）至关重要。VirE1是VirE2的分泌伴侣；它可以阻止VirE2形成聚集体，并阻止其与细菌细胞中的t链结合。因此，我们假设在植物细胞中表达足够数量的VirE1可能通过阻止VirE2结合t链来阻断T-DNA的传播。本研究表明，表达virE1的拟南芥植株的根外植体形成的肿瘤比不表达virE1的植株的根外植体少3.5倍。此外，这种抗性对vire2介导的农杆菌转化具有特异性。经过基因改造的植物可能比生物防治更有效。
Crown gall disease, caused by Agrobacterium tumefaciens, remains a serious agricultural problem despite current biocontrol methods. Agrobacterium tumefaciens transfers single-stranded DNA (T-strands) into plant cells along with several virulence proteins, including a: single-stranded DNA-binding protein (VirE2). In plant cells, T-strands are protected from nucleases and targeted to the nucleus by VirE2, which is essential for efficient transmission (transfer and integration) of T-strands. VirE1 is the secretory chaperone for VirE2; it prevents VirE2 from forming aggregates and from binding the T-strands in bacteria] cells. Therefore, we hypothesized that sufficient quantities of VirE1 expressed in plant cells might block T-DNA transmission by preventing VirE2 from binding T-strands. Here we show that root explants from Arabidopsis thaliana plants that expressed virE1 formed 3.5-fold fewer tumors than roots from plants without virE1. Also, this resistance was specific for VirE2-mediated Agrobacterium transformation. Plants that have been genetically altered to resist crown gall may prove more effective than biological control.