Premature remodeling of fat body and fat mobilization triggered by platelet-derived growth factor/VEGF receptor in Drosophila
Premature remodeling of fat body and fat mobilization triggered by platelet-derived growth factor/VEGF receptor in Drosophila
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血小板源性生长因子/VEGF受体触发果蝇脂肪体过早重塑和脂肪动员
DOI：
10.1096/fj.201601127r
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发表时间：
2017
期刊：
影响因子：
4.8
通讯作者：
Yang Xiaohang
中科院分区：
文献类型：
--
作者：
Zheng Huimei;Wang Xuexiang;Guo Pengfei;Ge Wanzhong;Yan Qinfeng;Gao Weiqiang;Xi Yongmei;Yang Xiaohang
在果蝇中，伴随脂肪动员的脂肪体重塑是蜕皮激素诱导的动态过程，仅发生在变态期间。在这里，我们发现激活的果蝇血小板衍生生长因子/VEGF受体（PVR）足以诱导脂肪体的形状变化，从紧密结合的多边形细胞薄层到解聚的圆形细胞簇。这些形态学变化让人想起在脂肪体重塑开始后的早期化蛹期间所看到的那些。PVR的激活还触发脂解的早期发作和内部储存的动员，如小脂滴的出现和脂解相关基因的上调所揭示的。我们发现PVR在脂肪体中显示出动态表达模式，并在蜕皮激素信号控制下在幼虫-预蛹过渡期达到峰值。去除PVR虽然不能阻止蜕皮激素诱导的脂肪体重塑，但会导致蜕皮激素信号上调。我们的数据表明，PVR在双重保护机制中发挥作用，该机制涉及蜕皮激素诱导的脂肪体重塑途径和增强的PVR途径，以实现有效的脂质动员。激活的c-kit（果蝇脂肪体中PVR的小鼠同源物）的异位表达也导致了类似的表型。这可能表明c-kit的一种新功能，因为它与哺乳动物的脂质代谢有关。郑洪，王，X.，Guo，P.，中国科学院院士，乔治，W.，阎青，越-地高文，Xi，Y.，Yang，X.果蝇中血小板衍生生长因子/VEGF受体引发的脂肪体过早重塑和脂肪动员。FASEB J. 31，1964-1975（2017）。www.fasebj.org
In Drosophila, fat‐body remodeling accompanied with fat mobilization is an ecdysone‐induced dynamic process that only occurs during metamorphosis. Here, we show that the activated Drosophila platelet‐derived growth factor/VEGF receptor (PVR) is sufficient to induce shape changes in the fat body, from thin layers of tightly conjugated polygonal cells to clusters of disaggregated round‐shaped cells. These morphologic changes are reminiscent of those seen during early pupation upon initiation of fat‐body remodeling. Activation of PVR also triggers an early onset of lipolysis and mobilization of internal storage, as revealed by the appearance of small lipid droplets and up‐regulated lipolysis‐related genes. We found that PVR displays a dynamic expression pattern in the fat body and peaks at the larval‐prepupal transition under the control of ecdysone signaling. Removal of PVR, although it does not prevent ecdysone‐induced fat‐body remodeling, causes ecdysone signaling to be up‐regulated. Our data reveal that PVR is active in a dual‐secured mechanism that involves an ecdysone‐induced fat‐body remodeling pathway and a reinforced PVR pathway for effective lipid mobilization. Ectopic expression of activated c‐kit—the mouse homolog of PVR in the Drosophila fat body—also results in a similar phenotype. This may suggest a novel function of c‐kit as it relates to lipid metabolism in mammals.—Zheng, H., Wang, X., Guo, P., Ge, W., Yan, Q., Gao, W., Xi, Y., Yang, X. Premature remodeling of fat body and fat mobilization triggered by platelet‐derived growth factor/VEGF receptor in Drosophila. FASEB J. 31, 1964–1975 (2017). www.fasebj.org