Acoustic activation of drug delivery from polymeric micelles: effect of pulsed ultrasound
Acoustic activation of drug delivery from polymeric micelles: effect of pulsed ultrasound
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DOI：
10.1016/s0168-3659(01)00216-4
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发表时间：
2001-04-28
影响因子：
10.8
通讯作者：
Rapoport, N
中科院分区：
文献类型：
--
作者：
Marin, A;Muniruzzaman, M;Rapoport, N
研究了连续波（CW）和脉冲20 khz超声对HL-60细胞从磷酸盐缓冲盐水（PBS）和Pluronic胶束溶液中摄取多柔比星（DOX）的影响。连续波和脉冲超声都能增强PBS和Pluronic胶束对DOX的吸收。影响药物摄取的主要因素是超声功率密度；然而，随着功率的增加，药物摄取的增强伴随着广泛的细胞声溶。对于PBS，超声脉冲持续时间和脉冲间隔时间对药物摄取没有明显影响。对于Pluronic胶束，在总超声时间相同的情况下，随着脉冲持续时间的增加，吸收量增加。对于2s脉冲，摄取接近连续波超声。脉冲间隔时间对Pluronic胶束的药物摄取无显著影响。脉冲持续时间对药物摄取影响的数据表明，药物从胶束释放的特征时间和细胞对药物的摄取具有可比性。结果指出两种独立的机制控制Pluronic胶束对药物摄取的声激活。这两种机制协同工作。第一个与声触发的药物从胶束释放有关，这导致培养液中游离药物的浓度更高。第二种机制是基于细胞膜的扰动，导致胶束包裹药物的摄取增加。荧光显微镜证实了Pluronic胶束在细胞内的摄取。(C) 2001年Elsevier Science B.V.出版
The effect of a continuous wave (CW) and pulsed 20-kHz ultrasound on the Doxorubicin (DOX) uptake by HL-60 cells from the phosphate buffered saline solution (PBS) and Pluronic micellar solutions was studied. Both CW and pulsed ultrasound enhanced DOX uptake from PBS and Pluronic micelles. The main factor that effected drug uptake was ultrasound power density; however, with increasing power, the enhanced drug uptake was accompanied by the extensive cell sonolysis. For PBS, no significant effect of duration of the ultrasound pulse or inter-pulse interval on the drug uptake was observed. For Pluronic micelles, the uptake increased with increasing pulse duration in the range 0.1-2 s, overall sonication time being the same. For 2-s pulses, the uptake was close to that under CW ultrasound. There was no significant effect of the duration of the inter-pulse interval on the drug uptake from Pluronic micelles. The data on the effect of pulse duration on drug uptake suggest that the characteristic times of drug release from micelles and drug uptake by the cells are comparable. The results point to two independent mechanisms controlling acoustic activation of drug uptake from Pluronic micelles. Both mechanisms work in concert. The first one is related to the acoustically-triggered drug release from micelles that results ill higher concentration of the free drug in the incubation medium. The second mechanism is based on the perturbation of cell membranes that results in the increased uptake of the micellar-encapsulated drug. The intracellular uptake of Pluronic micelles was confirmed by fluorescence microscopy. (C) 2001 Published by Elsevier Science B.V.