The effect of phosphorylcholine-coated materials on the inflammatory response and fibrous capsule formation:: In vitro and in vivo observations
The effect of phosphorylcholine-coated materials on the inflammatory response and fibrous capsule formation:: In vitro and in vivo observations
复制标题
DOI：
10.1002/jbm.a.10141
复制
发表时间：
2004-01-01
影响因子：
4.9
通讯作者：
Lloyd, AW
中科院分区：
文献类型：
--
作者：
Goreish, HH;Lewis, AL;Lloyd, AW
进行了几个实验以比较人巨噬细胞和粒细胞炎性细胞与聚对苯二甲酸乙二醇酯基底和涂覆有磷酸胆碱（PC）基聚合物的相同基底的体外粘附。在试验的不同阶段纳入各种类型的血清表明，表面的蛋白质吸附和钝化可能是减少粘附在未涂层聚对苯二甲酸乙二醇酯对照品上的炎性细胞数量的原因。在进行的所有测定中，粘附到PC包被的样品上的炎性细胞的数量在统计学上显著减少（p < 0.05;方差分析），这与这些表面抵抗蛋白质粘附的能力有关。在家兔肌内模型中植入PC涂层不锈钢和高密度聚乙烯USP对照样品。植入后4周，对植入部位周围存在的炎性细胞数量进行组织学检查，结果显示，与对照组相比，PC涂层样品相关的细胞减少了40%，但无统计学显著性。然而，植入后13周，PC涂层样本的纤维囊厚度几乎完全消退，而4周时略有减少，并且在统计学上比高密度聚乙烯USP对照更薄（p < 0.01; Mann-Whitney U检验）。这些发现支持了以下观点：基于PC的技术在体外观察到的低生物相互作用可导致体内炎症和异物反应减少。(C)2003 Wiley Periodicals，Inc.
Several experiments were performed to compare the in vitro adhesion of human macrophage and granulocyte inflammatory cells to polyethylene terephthalate substrate and the same coated with a phosphorylcholine (PC)-based polymer. The inclusion of various types of serum at different stages in the assay indicated that protein adsorption and passivation of the surface may be responsible for reducing the number of inflammatory cells adhering to the uncoated polyethylene terephthalate controls. In all of the assays performed there was a statistically significant reduction (p < 0.05; analysis of variance) of the number of inflammatory cells adhering to the PC-coated samples, linked to the ability of these surfaces to resist protein adhesion. Implantations of PC-coated stainless steel and high-density polyethylene USP control samples were made in a rabbit intramuscular model. Histological examination of the number of inflammatory cells present around the implant sites 4 weeks postimplantation showed there were 40% less cells associated with the PC-coated samples compared with control, but this was not statistically significant. Fibrous capsule thickness, however, whereas marginally less at 4 weeks, had almost completely regressed for the PC-coated sample at 13 weeks postimplantation and was statistically thinner (p < 0.01; Mann-Whitney U test) than the high-density polyethylene USP control. These findings support the view that low biointeractions observed for PC-based technology in vitro can result in reduced inflammation and foreign body reaction in vivo. (C) 2003 Wiley Periodicals, Inc.