NEW MODEL OF BILATERAL HEMISPHERIC ISCHEMIA IN THE UNANESTHETIZED RAT
NEW MODEL OF BILATERAL HEMISPHERIC ISCHEMIA IN THE UNANESTHETIZED RAT
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DOI：
10.1161/01.str.10.3.267
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发表时间：
1979-01-01
期刊：
影响因子：
8.3
通讯作者：
BRIERLEY, JB
中科院分区：
文献类型：
--
作者：
PULSINELLI, WA;BRIERLEY, JB
本文介绍了一种新的非麻醉大鼠短暂性双侧大脑半球缺血模型。在乙醚麻醉期间，通过第1颈椎的椎间孔电灼大鼠的椎动脉，并在颈总动脉周围松散地放置可逆卡环。24 h后，将清醒大鼠束缚，颈动脉环收紧，造成4支血管闭塞。分别于10、20或30 min后取颈动脉卡环，阻断4支血管，72 h后灌注固定处死动物。将在缺血或缺血后期间惊厥的大鼠从研究中排除。所有大鼠进行20或30分钟的4血管闭塞显示缺血性神经元损伤。H1和旁正中海马、纹状体和后新皮层的第3、5和6层是最常受损的区域。该模型的优点是易于制备许多动物，可预测的缺血性神经元损伤的高比率，癫痫发作的低发生率和没有麻醉。
A new model of transient, bilateral hemispheric ischemia in the unanesthetized rat is described. During ether anesthesia, the rat''s vertebral arteries were electrocauterized through the alar foramina of the 1st cervical vertebra and reversible clasps placed loosely around the common carotid arteries. After 24 h, the awake rats were restrained and the carotid clasps tightened to produce 4 vessel occlusion. The carotid clasps were removed after 10, 20 or 30 min for 4 vessel occlusion and the animals killed by perfusion fixation 72 h later. Rats which convulsed during the ischemic or post-ischemic period were excluded from study. All rats subjected to 20 or 30 min of 4 vessel occlusion showed ischemic neuronal damage. The H1 and paramedian hippocampus, striatum and layers 3, 5 and 6 of the posterior neocortex were the regions most frequently damaged. The advantages of the model are the ease of preparation of many animals, a high rate of predictable ischemic neuronal damage, a low incidence of seizures and the absence of anesthesia.