Metabolic changes in mouse sperm during capacitation
Metabolic changes in mouse sperm during capacitation
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DOI：
10.1093/biolre/ioaa114
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发表时间：
2020-10-01
影响因子：
3.6
通讯作者：
Buck, Jochen
中科院分区：
文献类型：
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作者：
Balbach, Melanie;Gervasi, Maria Gracia;Buck, Jochen
哺乳动物的精子在休眠状态下储存在附睾处。射精后，它们必须立即开始产生足够的能量来维持运动和支持获能。虽然在获能过程中这种能量需求的增加是确定的，但仍不清楚小鼠精子如何改变其新陈代谢来满足这一需求。我们现在证明，使小鼠精子获能增强葡萄糖摄取，将葡萄糖摄取确定为获能的一个功能标志。使用胞外通量分析仪，我们发现在获能过程中糖酵解和氧化磷酸化增加。此外，这种氧化磷酸化的增加依赖于糖酵解，这为小鼠精子的糖酵解和氧化磷酸化之间的联系提供了实验证据。
Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm.