Retention of the label during the conversion of [3-3H] squalene into (3S)-2,3-oxidosqualene catalyzed by mammalian squalene oxidase.
Retention of the label during the conversion of [3-3H] squalene into (3S)-2,3-oxidosqualene catalyzed by mammalian squalene oxidase.
复制标题
在哺乳动物角鲨烯氧化酶的催化下，[3-3H]角鲨烯转化为(3S)-2,3-氧化角鲨烯期间标记的保留。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
F. Schuber
中科院分区：
文献类型：
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作者：
O. Boutaud;M. Ceruti;L. Cattel;F. Schuber
角鲨烯环氧酶是唯一已知的催化烯烃环氧化的黄素蛋白。为了测试基于非血红素金属的催化机制的可能性，研究了通过部分纯化的猪肝角鲨烯环氧酶将化学合成的[3- 3 H]角鲨烯转化为[3 H] 2，3-氧化角鲨烯。没有观察到标记氢的交换，排除了涉及例如，C-3位的铁卡宾类型物质。
Squalene epoxidase is the only known flavoprotein that catalyzes the epoxidation of an olefin. In order to test the possibility of a catalytic non-heme metal-based mechanism, the conversion of chemically synthesized [3-3H]squalene into [3H]2,3-oxidosqualene, by partially purified pig liver squalene epoxidase, was studied. No exchange of the labeled hydrogen could be observed, ruling out a mechanism involving, e.g., an iron carbene type species at C-3.