Systemic effects of dietary n-3 PUFA supplementation accompany changes of CNS parameters in cerebral hypoperfusion
Systemic effects of dietary n-3 PUFA supplementation accompany changes of CNS parameters in cerebral hypoperfusion
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DOI：
10.1111/j.1749-6632.2002.tb04800.x
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发表时间：
2002-01-01
期刊：
影响因子：
--
通讯作者：
Luiten, PGM
中科院分区：
文献类型：
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作者：
Farkas, E;DeWilde, MC;Luiten, PGM
膳食补充长链多不饱和脂肪酸（PUFAs）已成为缓解或预防脑血管病理生理的一种有吸引力的可能性。为了表征鱼油中主要存在的n-3 pufa对脑血管的潜在有益作用，我们建立了一个实验范式，给慢性脑灌注不足的大鼠提供富含n-3 pufa的饮食。脑灌注不足是由4月龄大鼠颈总动脉（2VO）永久性双侧闭塞造成的，存活时间为3个月。同时，从断奶至实验结束均饲喂实验饲粮。对照饲料与标准大鼠饲料相当，而饲料1含有额外的n-3 PUFAs，饲料2进一步富含结构磷脂和神经递质前体。综上所述，数据显示，饮食2改善了Morris水迷宫中2VO大鼠的空间学习。饮食1和饮食2均增强了血脑屏障参数，增加了海马m1型毒蕈碱胆碱能受体的密度，与脑灌注率无关。除了对这些结果的概述外，还介绍了支持或伴随CNS描述的变化。简而言之，血浆皮质酮浓度升高最明显的是2VO，而肝脏和脾脏的相对重量则因饲料而增加。这些数据引起了人们对慢性补充富含n-3 pufa的饮食不仅在中枢神经系统，而且在外周神经系统的变化的关注。
Dietary supplementation with long-chain polyunsaturated fatty acids (PUFAs) has become an attractive possibility to alleviate or prevent cerebrovascular pathophysiology. To characterize the potentially beneficial cerebrovascular action of n-3 PUFAs that predominantly occur in fish oil, we set up an experimental paradigm where rats with chronic cerebral hypo-perfusion were supplied with n-3 PUFA-enriched diets. Cerebral hypoperfusion was created by a permanent, bilateral occlusion of the common carotid arteries (2VO) of rats at the age of 4 months, with a survival of 3 months. Simultaneously, the rats were provided with experimental diets from the time of weaning until the termination of the experiments. The control diet was comparable to standard rat chow, while diet 1 contained additional n-3 PUFAs and diet 2 was further enriched with structural phospholipids and neurotransmitter precursors. In summary, the data show that diet 2 improved spatial learning of 2VO rats in the Morris water maze. Both diet 1 and diet 2 augmented blood-brain barrier parameters and increased the density of the M1-type muscarinic cholinergic receptors in the hippocampus independent of the rate of cerebral perfusion. In addition to an overview of these results, changes that were supportive or accompaning those described in the CNS are also presented. Briefly, plasma corticosterone concentration was elevated most explicitly by 2VO, while the relative weight of the liver and spleen increased due to the diets. The data draw attention to changes not only in the CNS, but also in the periphery as a consequence of chronic supplementation with n-3 PUFA-enriched diets.