High-contrast imaging of plastic-embedded tissues by phase contrast electron microscopy
High-contrast imaging of plastic-embedded tissues by phase contrast electron microscopy
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DOI：
10.1093/jmicro/dfp004
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发表时间：
2009-04-01
期刊：
影响因子：
--
通讯作者：
Nagayama, Kuniaki
中科院分区：
文献类型：
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作者：
Atsuzawa, Kimie;Usuda, Nobuteru;Nagayama, Kuniaki
相衬电子显微镜利用相位板已被认为是适合于弱相位物体的高对比度观察。这一新的技术是新应用于组织化学染色强相对象的锇化生物标本。用Zernike和Hilbert成像模式的相差电子显微镜观察不同厚度的切片，通过使用重金属Zn和Os的ZIO技术对高尔基体进行专门染色。在真实的空间中的图像对比度和傅立叶空间中的功率谱的定量分析表明，即使对于强相位对象，也有高对比度增益。这一结果清楚地表明，相衬电子显微镜不仅可以有效地用于弱相位物体，而且还可以用于生物学中的强相位物体。
Phase contrast electron microscopy utilizing phase plates has been considered suitable for high-contrast observation of weak phase objects. This novel technique was newly applied to histochemically stained strong phase objects of osmificated biological specimens. Sections of various thicknesses, specifically stained for the Golgi apparatus by the ZIO technique using the heavy metals Zn and Os, were observed with a phase contrast electron microscope in Zernike and Hilbert imaging modes. Quantitative analysis of image contrast in real space and the power spectrum in Fourier space showed a high-contrast gain even for strong phase objects. This result clearly indicates that phase contrast electron microscopy can be effectively used not only for weak phase objects but also for strong phase objects in biology.