The Neuroprotective Effect of Curcumin and Nigella sativa Oil Against Oxidative Stress in the Pilocarpine Model of Epilepsy: A Comparison with Valproate
The Neuroprotective Effect of Curcumin and Nigella sativa Oil Against Oxidative Stress in the Pilocarpine Model of Epilepsy: A Comparison with Valproate
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DOI：
10.1007/s11064-011-0544-9
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发表时间：
2011-11-01
影响因子：
4.4
通讯作者：
Noor, Neveen A.
中科院分区：
文献类型：
--
作者：
Ezz, Heba S. Aboul;Khadrawy, Yasser A.;Noor, Neveen A.
氧化应激已被认为在癫痫发生和匹罗卡品诱发的癫痫发作中起作用。本研究旨在探讨姜黄素、黑草油（NSO）和丙戊酸对匹罗卡品处理大鼠海马中丙二醛、一氧化氮、还原性谷胱甘肽水平及过氧化氢酶、Na+、K+- atp酶和乙酰胆碱酯酶活性的抗氧化作用。匹罗卡品诱导癫痫动物模型，静置22 d，建立癫痫慢性期。然后用姜黄素、NSO或丙戊酸治疗21天。结果显示，匹罗卡匹化大鼠海马区存在氧化应激，一氧化氮水平升高，谷胱甘肽水平和过氧化氢酶活性降低。此外，匹罗卡品后海马Na+、K+- atp酶活性降低，乙酰胆碱酯酶活性升高。姜黄素、NSO或丙戊酸治疗改善了匹罗卡平引起的大部分变化，并使海马中的Na+、K+- atp酶活性恢复到控制水平。本研究反映了姜黄素和NSO在降低癫痫动物的氧化应激、兴奋性和诱导癫痫发作以及改善抗癫痫药物的一些不良反应方面具有良好的抗惊厥和抗氧化作用。
Oxidative stress has been implicated to play a role in epileptogenesis and pilocarpine-induced seizures. The present study aims to evaluate the antioxidant effects of curcumin, Nigella sativa oil (NSO) and valproate on the levels of malondialdehyde, nitric oxide, reduced glutathione and the activities of catalase, Na+, K+-ATPase and acetylcholinesterase in the hippocampus of pilocarpine-treated rats. The animal model of epilepsy was induced by pilocarpine and left for 22 days to establish the chronic phase of epilepsy. These animals were then treated with curcumin, NSO or valproate for 21 days. The data revealed evidence of oxidative stress in the hippocampus of pilocarpinized rats as indicated by the increased nitric oxide levels and the decreased glutathione levels and catalase activity. Moreover, a decrease in Na+, K+-ATPase activity and an increase in acetylcholinesterase activity occurred in the hippocampus after pilocarpine. Treatment with curcumin, NSO or valproate ameliorated most of the changes induced by pilocarpine and restored Na+, K+-ATPase activity in the hippocampus to control levels. This study reflects the promising anticonvulsant and potent antioxidant effects of curcumin and NSO in reducing oxidative stress, excitability and the induction of seizures in epileptic animals and improving some of the adverse effects of antiepileptic drugs.