Textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies
Textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies
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DOI：
10.1016/j.jhazmat.2010.11.049
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发表时间：
2011-02-15
影响因子：
13.6
通讯作者：
Jadhav, Jyoti P.
中科院分区：
文献类型：
--
作者：
Phugare, Swapnil S.;Kalyani, Dayanand C.;Jadhav, Jyoti P.
本研究旨在使用两种细菌培养物评估已开发的微生物群落 SDS 的 Red HE3B 降解潜力。普罗维登西亚sp。 SDS (PS) 和铜绿假单胞菌菌株 BCH (PA) 最初是从染料污染的土壤中分离出来的。研究发现，与单个菌株相比，联合体对 Red HE3B 的脱色和降解速度要快得多。这些菌株的强烈代谢活性导致Red HE3B (50 mg l(-1))在1小时内100%脱色。显着诱导各种染料脱色酶，即。藜芦醇氧化酶、漆酶、偶氮还原酶和 DCIP 还原酶与对照相比，表明它们参与了整个脱色和降解过程。 HPLC、FTIR 和 GC-MS 等分析研究用于仔细检查生物降解过程。使用洋葱根细胞对微生物处理前后的毒理学研究进行了细胞毒性、遗传毒性、氧化应激、抗氧化酶状态、蛋白质氧化和脂质过氧化分析。 A. cepa 的毒性分析表明，染料 Red HE3B 对根细胞产生氧化应激和随后的毒性作用，而染料的生物降解代谢物本质上毒性相对较小。植物毒性研究还表明微生物处理有利于 Red HE3B 的解毒。 (C) 2010 Elsevier B.V. 保留所有权利。
The present study aims to evaluate Red HE3B degrading potential of developed microbial consortium SDS using two bacterial cultures viz. Providencia sp. SDS (PS) and Pseudomonas aeuroginosa strain BCH (PA) originally isolated from dye contaminated soil. Consortium was found to be much faster for decolorization and degradation of Red HE3B compared to the individual bacterial strain. The intensive metabolic activity of these strains led to 100% decolorization of Red HE3B (50 mg l(-1)) with in 1 h. Significant induction of various dye decolorizing enzymes viz. veratryl alcohol oxidase, laccase, azoreductase and DCIP reductase compared to control, point out towards their involvement in overall decolorization and degradation process. Analytical studies like HPLC, FTIR and GC-MS were used to scrutinize the biodegradation process. Toxicological studies before and after microbial treatment was studied with respect to cytotoxicity, genotoxicity, oxidative stress, antioxidant enzyme status, protein oxidation and lipid peroxidation analysis using root cells of Allium cepa. Toxicity analysis with A. cepa signifies that dye Red HE3B exerts oxidative stress and subsequently toxic effect on the root cells where as biodegradation metabolites of the dye are relatively less toxic in nature. Phytotoxicity studies also indicated that microbial treatment favors detoxification of Red HE3B. (C) 2010 Elsevier B.V. All rights reserved.