Plasma dipeptidyl peptidase-IV activity in patients with type-2 diabetes mellitus correlates positively with HbAlc levels, but is not acutely affected by food intake
Plasma dipeptidyl peptidase-IV activity in patients with type-2 diabetes mellitus correlates positively with HbAlc levels, but is not acutely affected by food intake
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DOI：
10.1530/eje.1.02221
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发表时间：
2006-09-01
影响因子：
5.8
通讯作者：
Vilsboll, Tina
中科院分区：
文献类型：
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作者：
Ryskjaer, Jakob;Deacon, Carolyn F.;Vilsboll, Tina
目的：胰高血糖素样肽-1 (GLP-1) 和葡萄糖依赖性促胰岛素多肽是肠促胰岛素激素，响应膳食摄入而分泌。肠促胰岛素激素刺激胰岛素分泌，对于维持正常血浆葡萄糖浓度至关重要。两种肠促胰岛素激素均被二肽基肽酶-IV (DPP-IV) 快速代谢。众所周知，2型糖尿病患者的肠促胰岛素作用受损。因此，本研究的目的是调查 2 型糖尿病患者和对照受试者在空腹和餐后状态下的血浆 DPP-IV 活性。 设计：该研究包括两个方案。方案一涉及 40 名禁食 2 型糖尿病患者（28 名男性）；年龄 61 +/- 1.4（平均+/- S.E.M.）岁；体重指数 (BMI) 31 +/- 0.6kg/m(2)；糖化血红蛋白 7.2 +/- 0.2%；并对 20 名匹配的对照对象（14 名男性）进行了研究。方案二涉及八名 2 型糖尿病患者（六名男性）； 63岁+/-1.2岁；体重指数 33 +/- 0.5 公斤/米 (2)；糖化血红蛋白 7.5 +/- 0.4%；纳入了八名匹配的对照受试者。方法：在方案一中，评估了 DPP-IV 活性的空腹值，在方案二中，估计了标准膳食测试（5 6 6 kcal）期间的餐后 DPP-IV 活性。结果：与对照组受试者相比，该患者组的平均空腹血浆 DPP-IV 活性（表示为 GLP-1 的降解）显着较高（67.5 +/- 1.9 vs 56.8 +/- 2.2 fmol） GLP-1/h（平均值+S.E.M.）；P=0.001）。在2型糖尿病患者中，DPP-IV活性与FPG和HbAlc呈正相关，与糖尿病病程和患者年龄呈负相关。各组血浆 DPP-IV 活性均未见餐后变化。结论：空腹状态下血浆 DPP-IV 活性增加，且与 FPG 和 HbAlc 水平呈正相关，但进餐和血糖急剧变化后血浆 DPP-IV 活性不发生改变。
Objective: Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide are incretin hormones, secreted in response to meal ingestion. The incretin hormones stimulate insulin secretion and are essential for the maintenance of normal plasma glucose concentrations. Both incretin hormones are metabolized quickly by the enzyme dipeptidyl peptidase-IV (DPP-IV). It is well known that type-2 diabetic patients have an impaired incretin effect. Therefore, the aim of the present study was to investigate plasma DPP-IV activity in the fasting and the postprandial state in type-2 diabetic patients and control subjects.Design: The study included two protocols. Protocol one involved 40 fasting type-2 diabetic patients (28 men); age 61 +/- 1.4 (mean +/- S.E.M.) years; body mass index (BMI) 31 +/- 0.6kg/m(2); HbAlc 7.2 +/- 0.2%; and 20 matched control subjects (14 men) were studied. Protocol two involved eight type 2 diabetic patients (six men); age 63 +/- 1.2 years; BMI 33 +/- 0.5kg/m(2); HbAlc 7.5 +/- 0.4%; eight matched control subjects were included.Methods: In protocol one, fasting values of DPP-IV activity were evaluated and in protocol two, postprandial DPP-IV activity during a standard meal test (5 6 6 kcal) was estimated.Results: Mean fasting plasma DPP-IV activity (expressed as degradation of GLP-1) was significantly higher in this patient group compared with the control subjects (67.5 +/- 1.9 vs 56.8 +/- 2.2 fmol GLP-1/h (mean+S.E.M.); P=0.001). In the type-2 diabetic patients, DPP-IV activity was positively correlated to FPG and HbAlc and negatively to the duration of diabetes and age of the patients. No postprandial changes were seen in plasma DPP-IV activity in any of the groups.Conclusions: Plasma DPP-IV activity increases in the fasting state and is positively correlated to FPG and HbAlc levels, but plasma DPP-IV activity is not altered following meal ingestion and acute changes in plasma glucose.