Genome analysis of the proteorhodopsin-containing marine bacterium Polaribacter sp MED152 (Flavobacteria)
Genome analysis of the proteorhodopsin-containing marine bacterium Polaribacter sp MED152 (Flavobacteria)
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DOI：
10.1073/pnas.0712027105
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发表时间：
2008-06-24
影响因子：
11.1
通讯作者：
Pedros-Alio, Carlos
中科院分区：
文献类型：
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作者：
Gonzalez, Jose M.;Fernandez-Gomez, Beatriz;Pedros-Alio, Carlos
对海洋蓝藻和蛋白质藻基因组的分析为深入了解这些生物的生活策略以及它们的生态型分化和代谢提供了基础。然而，对第三大浮游细菌类群类杆菌的可比性分析仍然缺乏。本文对Polaribacter sp.的基因组进行了研究。菌株MED152。一方面，MED152含有大量与表面或颗粒附着、滑动运动和聚合物降解有关的基因。这与目前假设的海洋拟杆菌的生命策略是一致的。另一方面，它含有蛋白质结合蛋白基因，以及一套非凡的感知和响应光的基因，这可能会在营养不良的阳光照耀的海洋表面寻找新的颗粒来定居时提供生存优势。此外，光照下二氧化碳固定的增加表明，有限的中枢代谢得到了无机碳固定的补充。这是由一种独特的膜转运蛋白和羧基酶的组合来调节的。这表明了一种双重生命策略，如果实验证实，这种策略将与表层海洋中另外两个主要细菌群(自养蓝藻和异养蛋白细菌)的已知情况截然不同。Polaribacter基因组提供了对含有蛋白视紫红质细菌的生理能力的见解。基因组将作为一个模型，研究细菌中表达蛋白质结合蛋白的细胞和分子过程，它们对海洋环境的适应，以及它们在碳循环中的作用。
Analysis of marine cyanobacteria and proteobacteria genomes has provided a profound understanding of the life strategies of these organisms and their ecotype differentiation and metabolisms. However, a comparable analysis of the Bacteroidetes, the third major bacterioplankton group, is still lacking. In the present paper, we report on the genome of Polaribacter sp. strain MED152. On the one hand, MED152 contains a substantial number of genes for attachment to surfaces or particles, gliding motility, and polymer degradation. This agrees with the currently assumed life strategy of marine Bacteroidetes. On the other hand, it contains the proteorhoclopsin gene, together with a remarkable suite of genes to sense and respond to light, which may provide a survival advantage in the nutrient-poor sun-lit ocean surface when in search of fresh particles to colonize. Furthermore, an increase in CO2 fixation in the light suggests that the limited central metabolism is complemented by anaplerotic inorganic carbon fixation. This is mediated by a unique combination of membrane transporters and carboxylases. This suggests a dual life strategy that, if confirmed experimentally, would be notably different from what is known of the two other main bacterial groups (the autotrophic cyanobacteria and the heterotrophic proteobacteria) in the surface oceans. The Polaribacter genome provides insights into the physiological capabilities of proteorhodopsin-containing bacteria. The genome will serve as a model to study the cellular and molecular processes in bacteria that express proteorhoclopsin, their adaptation to the oceanic environment, and their role in carbon-cycling.