Mucin-Inspired Lubrication on Hydrophobic Surfaces
Mucin-Inspired Lubrication on Hydrophobic Surfaces
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DOI：
10.1021/acs.biomac.7b00605
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发表时间：
2017-08-01
影响因子：
6.2
通讯作者：
Lieleg, Oliver
中科院分区：
文献类型：
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作者：
Kaesdorf, Benjamin T.;Weber, Florian;Lieleg, Oliver
在人体中，称为粘蛋白的高分子量糖蛋白在保护上皮表面免受病原体攻击、控制分子向组织的通过以及实现具有非常低的摩擦系数的边界润滑方面发挥关键作用。然而，这些生物大分子参与这些基本过程的分子机制和化学基序都没有完全理解。因此，确定使粘蛋白等生物大分子成为杰出边界润滑剂的关键特征，可以为创造多功能人工超润滑剂奠定基础。我们在这里证明了猪胃粘蛋白（MUCSAC）和人唾液粘蛋白（MUCSB）的疏水末端肽结构域的吸附和润滑疏水PDMS表面的过程中的重要性。这些粘蛋白的胰蛋白酶消化导致这些末端结构域的去除，这伴随着润滑性以及表面吸附的损失。我们发现，这种损失可以部分地通过将疏水苯基连接到粘蛋白大分子的糖基化中心部分来补偿。此外，我们证明了简单的生物多糖葡聚糖可以用疏水基团官能化，这赋予多糖在PDMS上的有效表面吸附和良好的润滑性。
In the human body, high-molecular-weight glycoproteins called mucins play a key role in protecting epithelial surfaces against pathogenic attack, controlling the passage of molecules toward the tissue and enabling boundary lubrication with very low friction coefficients. However, neither the molecular mechanisms nor the chemical motifs of those biomacromolecules involved in these fundamental processes are fully understood. Thus, identifying the key features that render biomacromolecules such as mucins outstanding boundary lubricants could set the stage for creating versatile artificial superlubricants. We here demonstrate the importance of the hydrophobic terminal peptide domains of porcine gastric mucin (MUCSAC) and human salivary mucin (MUCSB) in the processes of adsorbing to and lubricating a hydrophobic PDMS surface. Tryptic digestion of those mucins results in removal of those terminal domains, which is accompanied by a loss of lubricity as well as surface adsorption. We show that this loss can in part be compensated by attaching hydrophobic phenyl groups to the glycosylated central part of the mucin macromolecule. Furthermore, we demonstrate that the simple biopolysaccharide dextran can be functionalized with hydrophobic groups which confers efficient surface adsorption and good lubricity on PDMS to the polysaccharide.