Dynamic changes in the initial colonization of Actinomyces naeslundii and Streptococcus gordonii using a new animal model.
Dynamic changes in the initial colonization of Actinomyces naeslundii and Streptococcus gordonii using a new animal model.
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DOI：
10.7883/yoken.66.11
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发表时间：
2013
影响因子：
2.2
通讯作者：
Xi Zhang;H. Senpuku
中科院分区：
文献类型：
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作者：
Xi Zhang;H. Senpuku
内氏放线菌和戈登链球菌是口腔菌群的优势菌和初始定植菌。A. naeslundii和S.在唾液膜覆盖的牙齿表面上的戈登氏菌以及它们之间的相互作用在生物膜的形成中起重要作用。最近，我们报道了NOD/SCID.e2f1（-）小鼠是研究口腔生物膜形成的变形链球菌在牙齿表面的有用模型。在这项研究中，我们的目的是确定NOD/SCID.e2f1（-）小鼠是否可以用于研究A. naeslundii和S. gordonii。定殖A.接种前24 h喂饲1%蔗糖水的小鼠内氏疟原虫感染率高于喂饲1 h的小鼠。a.接种后30-90 min，混合接种的内氏菌定殖率低于单种接种，而接种后120-180 min，内氏菌定殖率高于单种接种。混合接种诱导了较好的定殖效果。gordonii比单种接种后60 - 180 min。多克隆和异硫氰酸荧光素标记的抗体染色细菌显示更好的定殖S。当在体内使用混合培养物时，NOD/SCID.e2f1（-）小鼠可用于研究A. naeslundii和S. gordonii。长期供应蔗糖水为A. naeslundii，这反过来又支持了S. gordonii。
Actinomyces naeslundii and Streptococcus gordonii are the predominant bacteria and initial colonizers of oral microflora. The binding of A. naeslundii and S. gordonii and the interaction between them on the salivary pellicle-coated tooth surface play an important role in the biofilm development. Recently, we reported that NOD/SCID.e2f1(-) mice are a useful model for studying oral biofilm formation by Streptococcus mutans on the tooth surface. In this study, we aimed to determine whether NOD/SCID.e2f1(-) mice can be used for studying oral colonization of A. naeslundii and S. gordonii. Colonization of A. naeslundii in mice fed with 1% sucrose water for 24 h before inoculation was higher than that among mice fed with sucrose water for 1 h. A. naeslundii colonization using mixed species-inoculation was lower than that using single-species inoculation 30-90 min after inoculation; however, the colonization was higher 120-180 min after inoculation. The mixed inoculation induced better colonization of S. gordonii than single-species inoculation 60-180 min after inoculation. Polyclonal and fluorescein isothiocyanate-labeled antibody stained bacteria showed better colonization of S. gordonii when a mixed culture is used in vivo. NOD/SCID.e2f1(-) mice were useful for studying the initial colonization of A. naeslundii and S. gordonii. Long-term supply of sucrose water creates a favorable environment for the initial colonization of A. naeslundii that, in turn, supports the colonization of S. gordonii.