Absence of Junctional Glutamate Receptor Clusters in Drosophila Mutants Lacking Spontaneous Transmitter Release
Absence of Junctional Glutamate Receptor Clusters in Drosophila Mutants Lacking Spontaneous Transmitter Release
复制标题
缺乏自发递质释放的果蝇突变体中缺乏连接谷氨酸受体簇
作者：
M. Saitoe;T. Schwarz;J. A. Umbach;C. Gundersen;Y. Kidokoro
小泡胞吐在神经末梢的结果是自发的微型突触电位的功能意义知之甚少。在这里，通过使用具有突触前功能特定缺陷的果蝇突变体，我们发现谷氨酸受体正常地聚集在保留自发递质分泌但失去响应动作电位释放递质的能力的突变体的神经肌肉接头处。与此相反，受体聚集是有缺陷的突变体，其中自发和诱发囊泡胞吐是缺席。因此，自发的囊泡胞吐作用似乎与发育过程中谷氨酸受体的聚集密切相关。
Little is known about the functional significance of spontaneous miniature synaptic potentials, which are the result of vesicular exocytosis at nerve terminals. Here, by usingDrosophila mutants with specific defects in presynaptic function, we found that glutamate receptors clustered normally at neuromuscular junctions of mutants that retained spontaneous transmitter secretion but had lost the ability to release transmitter in response to action potentials. In contrast, receptor clustering was defective in mutants in which both spontaneous and evoked vesicle exocytosis were absent. Thus, spontaneous vesicle exocytosis appears to be tightly linked to the clustering of glutamate receptors during development.