Genetic variability in the mitochondrial control region of the Japanese Rock Ptarmigan Lagopus mutus japonicus
Genetic variability in the mitochondrial control region of the Japanese Rock Ptarmigan Lagopus mutus japonicus
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DOI：
10.3838/jjo.50.53
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发表时间：
2001-05-01
期刊：
影响因子：
--
通讯作者：
Koike, Hiroko
中科院分区：
文献类型：
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作者：
Baba, Yoshiyuki;Fujimaki, Yuzo;Koike, Hiroko
以松鸡栖息地的落石松鸡(Lagopus Mutus)羽毛为材料，提取DNA，用松鸡特异的引物进行第二次聚合酶链式反应，扩增出清晰的条带。通过与鸡和鹌鹑的比较发现，松鸡和斑羚(Bonasa Bonasia)的线粒体控制区可以分为三个结构域：中央结构域中具有F、D、C盒和CSB-1的更保守的部分，以及更多的左右结构域的变异区。仅在左端441bp处发现两种单倍型：来自平田山4个地点的21个单倍型LM1和来自赤石山的1个单倍型LM2。用来自北海道的36只榛子松鸡对同一区域进行了分析，发现了21个替换，定义了21个单倍型，表明日本松鸡地区的遗传变异性很低。孢粉学研究表明，日本松林带是日本松林的主要栖息地，在早期的过热时期，松林带的规模可能会缩小。这样的自然环境变化可能会对罗克特尔米根种群造成瓶颈事件，导致非常低的遗传变异性。
Fallen Rock Ptarmigan (Lagopus mutus) feathers from bird habitats were used to extract DNA and produced a clear PCR band after the second PCR with grouse-specific primers. Comparison with chicken and quail revealed that the mitochondrial control regions of the Rock Ptarmigan and Hazel Grouse (Bonasa bonasia) can be divided into three domains: a more conserved segment of the central domain with the F, D, and C boxes and CSB-1, as well as more variable regions of the left and right domains. Only two haplotypes were found in the Japanese Rock Ptarmigan at the 441 bp of the left domain: haplotype LM1 in 21 samples from 4 localities in the Hida mountains and haplotype LM2 in 1 sample from the Akaishi mountains. The same region was analyzed using 36 individuals of Hazel Grouse from Hokkaido, which exhibited 21 substitutions defining 21 haplotypes, indicating that the Japanese Rock Ptarmigan area has very low genetic variability. Palynological study indicated Pinus pumila zone, where is main habitat of the Japanes Rock Ptarmigan, would have been scaled down during the early hypsithermal interval. Such a natural environmental change might cause a bottleneck event to the Rock Ptarmigan population, resulting very low genetic variability.