Essential role of Nrf2 in keratinocyte protection from UVA by quercetin
Essential role of Nrf2 in keratinocyte protection from UVA by quercetin
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DOI：
10.1016/j.bbrc.2009.06.136
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发表时间：
2009-09-11
影响因子：
3.1
通讯作者：
Ishii, Tetsuro
中科院分区：
文献类型：
--
作者：
Kimura, Shintarou;Warabi, Eiji;Ishii, Tetsuro
紫外线A （UVA）照射引起的细胞损伤大多与氧化应激有关。槲皮素是一种主要的天然多酚，可以保护细胞免受uva引起的损伤。在此，我们研究了这种保护的分子机制。槲皮素预处理能明显抑制UVA诱导的人角化细胞HaCaT细胞凋亡，显著提高转录因子Nrf2蛋白水平，诱导抗氧化基因表达，显著降低UVA照射后活性氧的产生。重要的是，下调Nrf2的表达大大减弱了这些有益的作用。因此，槲皮素主要通过增强抗氧化基因转录因子Nrf2的积累来提高细胞内抗氧化活性，从而保护细胞免受UVA损伤。(C) 2009爱思唯尔公司版权所有。
Much of the cell injury caused by ultraviolet A (UVA) irradiation is associated with oxidative stress. Quercetin is a major natural polyphenol that is known to protect cells from UVA-induced damage. Here, we investigated the molecular mechanism of this protection. Quercetin pretreatment Strongly suppressed UVA-induced apoptosis in human keratinocyte HaCaT cells, markedly increased protein levels of the transcription Factor Nrf2, induced the expression of antioxidative genes, and dramatically reduced the production of reactive oxygen species following UVA irradiation. Importantly, these beneficial effects were greatly attenuated by downregulating Nrf2 expression. Thus, quercetin protects cells from UVA damage mainly by elevating intracellular antioxidative activity via the enhanced accumulation of a transcription factor for antioxidant genes, Nrf2. (C) 2009 Elsevier Inc. All rights reserved.