Response of Astrocytes to Blood Exposure due to Shunt Insertion in vitro.
Response of Astrocytes to Blood Exposure due to Shunt Insertion in vitro.
复制标题
星形胶质细胞对体外分流插入引起的血液暴露的反应。
DOI：
10.1002/aic.17485
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Harris,CarolynA
中科院分区：
文献类型：
--
作者：
Zaranek,Mira;Arshad,Rooshan;Zheng,Kevin;Harris,CarolynA
在脑积水治疗中，脑室区破裂伴脑脊液中存在血液已被证明会增加分流导管阻塞，但发生这种情况的机制通常未知。使用定制的孵育室，我们在14天后免疫荧光测定了有和无血液的分流导管上的细胞附着和形态。暴露于血液的样品显示出显著增加的细胞附着（平均总细胞计数392.0 ± 317.1与对照组的94.7 ± 44.5，p < 0.0001）。胶质细胞酸性蛋白表达分析显示相似的趋势（854.4 ± 450.7 vs.对照组174.3 ± 116.5，p < 0.0001）。开发了一种体外模型，以代表血脑屏障通透性增加后星形胶质细胞暴露于血液。星形胶质细胞暴露于血液增加了细胞数量及其在分流器上的扩散。
The breakdown of the ventricular zone with the presence of blood in cerebrospinal fluid has been shown to increase shunt catheter obstruction in the treatment of hydrocephalus, but the mechanisms by which this occurs are generally unknown. Using a custom‐built incubation chamber, we immunofluorescently assayed cell attachment and morphology on shunt catheters with and without blood after 14 days. Samples exposed to blood showed significantly increased cell attachment (average total cell count 392.0 ± 317.1 vs. control of 94.7 ± 44.5,p< 0.0001). Analysis of the glial fibrillary acidic protein expression showed similar trends (854.4 ± 450.7 vs. control of 174.3 ± 116.5,p< 0.0001). An in vitro model was developed to represent the exposure of astrocytes to blood following an increase in blood–brain barrier permeability. Exposure of astrocytes to blood increases the number of cells and their spread on the shunt.