Synergistic effect of baicalein, wogonin and oroxylin A mixture: Multistep inhibition of the NF-κB signalling pathway contributes to an anti-inflammatory effect of Scutellaria root flavonoids
Synergistic effect of baicalein, wogonin and oroxylin A mixture: Multistep inhibition of the NF-κB signalling pathway contributes to an anti-inflammatory effect of Scutellaria root flavonoids
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黄芩素、汉黄芩素和木木素 A 混合物的协同作用：多步抑制 NF-κB 信号通路有助于黄芩根黄酮的抗炎作用
DOI：
10.1007/s11418-017-1111-8
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发表时间：
2018
影响因子：
3.3
通讯作者：
and Fumiyuki Kiuchi
中科院分区：
文献类型：
--
作者：
Tomofumi Shimizu;Nobuhiko Shibuya;Yuji Narukawa;Naohiro Oshima;Noriyasu Hada;and Fumiyuki Kiuchi
作为我们对麻黄碱生物碱提取物（EFE）的持续研究的一部分，我们确定了两种用于EFE生产过程质量控制的定量标记，并寻求建立成本效益高且简单的定量分析方法。采用液相色谱/高分辨率质谱法（LC/HRMS）对不同产地、不同采收年限的麻黄提取物进行了分析，发现了两种提取物共有的显著峰。这些峰被鉴定为vicenin-2(1)和isovitexin 2″-O-rhamnoside(2)。采用液相色谱/质谱等密度条件进行定量分析，结果表明，EFE中1和2的含量分别为0.140 ~ 0.146%和0.350 ~ 0.411%。我们认为1和2是EFE定量分析的质量控制标记。此外，我们定量分析了与1和2共有的苷元芹菜素(3)，发现1到3和2到3的转化因子分别为1.3和1.5。因此，我们认为3是定量测定EFE中1和2含量的二级标准。本研究得到的一系列结果将为EFE的质量控制提供参考。
As part of our continuing study of ephedrine alkaloids-free Ephedra Herb extract (EFE) in pursuit of its approval as a crude drug preparation, we identified two quantitative markers for the quality control of the manufacturing process of EFE and sought to establish cost-effective and simple methods for quantitative analyses. We analysed Ephedra Herb extracts grown in different habitats and collection years by liquid chromatography/high-resolution mass spectrometry (LC/HRMS) and detected two notable peaks common to each extract. These peaks were identified as vicenin-2 (1) and isovitexin 2″-O-rhamnoside (2). Quantitative analyses using the isocratic condition of LC/MS showed that the content percentages of1and2in EFE were 0.140–0.146% and 0.350–0.411%, respectively. We concluded that1and2were adequate quality control markers for quantitative analysis of EFE. Furthermore, we quantitatively analysed apigenin (3), an aglycon common to1and2, and found that the conversion factors of1to3and2to3were 1.3 and 1.5, respectively. Therefore, we concluded that3was a secondary standard for quantifying the contents of1and2in EFE. A series of results obtained from this study will be valuable for the quality control of EFE.