Epigenome microarray platform for proteome-wide dissection of chromatin-signaling networks.
Epigenome microarray platform for proteome-wide dissection of chromatin-signaling networks.
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DOI：
10.1371/journal.pone.0006789
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发表时间：
2009-08-26
期刊：
影响因子：
3.7
通讯作者：
Gozani O
中科院分区：
文献类型：
--
作者：
Bua DJ;Kuo AJ;Cheung P;Liu CL;Migliori V;Espejo A;Casadio F;Bassi C;Amati B;Bedford MT;Guccione E;Gozani O
Knowledge of protein domains that function as the biological effectors for diverse post-translational modifications of histones is critical for understanding how nuclear and epigenetic programs are established. Indeed, mutations of chromatin effector domains found within several proteins are associated with multiple human pathologies, including cancer and immunodeficiency syndromes. To date, relatively few effector domains have been identified in comparison to the number of modifications present on histone and non-histone proteins. Here we describe the generation and application of human modified peptide microarrays as a platform for high-throughput discovery of chromatin effectors and for epitope-specificity analysis of antibodies commonly utilized in chromatin research. Screening with a library containing a majority of the Royal Family domains present in the human proteome led to the discovery of TDRD7, JMJ2C, and MPP8 as three new modified histone-binding proteins. Thus, we propose that peptide microarray methodologies are a powerful new tool for elucidating molecular interactions at chromatin.
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影响因子：
4.8
作者：
Kachirskaia, Ioulia;Shi, Xiaobing;Gozani, Or
通讯作者：
Gozani, Or
影响因子：
56.9
作者：
Huang, Y;Fang, J;Xu, RM
通讯作者：
Xu, RM
影响因子：
16.8
作者：
Collins, Robert E.;Northrop, Jeffrey P.;Cheng, Xiaodong
通讯作者：
Cheng, Xiaodong
影响因子：
21.3
作者：
Schneider, R;Bannister, AJ;Kouzarides, T
通讯作者：
Kouzarides, T
DOI：
10.1073/pnas.0808470105
发表时间：
2008-10-14
影响因子：
11.1
作者：
Koh, Andrew S.;Kuo, Alex J.;Mathis, Diane
通讯作者：
Mathis, Diane