Microsatellite multiplex panels for population genetic analysis of red sea bream Pagrus major
Microsatellite multiplex panels for population genetic analysis of red sea bream Pagrus major
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DOI：
10.1007/s12562-012-0469-5
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发表时间：
2012-05-01
影响因子：
1.9
通讯作者：
Taniguchi, Nobuhiko
中科院分区：
文献类型：
--
作者：
Gonzalez, Enrique Blanco;Aritaki, Masaki;Taniguchi, Nobuhiko
本研究利用多重PCR技术对真鲷的20个微卫星标记进行扩增，以降低真鲷遗传分析的人力和物力成本。这些面板的有用性进行了验证，在200鱼收集在5个采样点。在5个标记上出现无效等位基因，从进一步分析中删除。其余15个微卫星位点表现出高水平的杂合性（（E）范围0.34-0.97，（O）范围0.32-1）和每个位点的等位基因数的宽范围（范围5-46）。将微卫星位点的数目从3个增加到10个和15个，提高了群体分化（ST）和Nei（S）遗传距离估计的面板的性能。本研究的结果证实了有用的基因分型大量的微卫星DNA标记，以扩大我们的知识，红鲷种群遗传学。
In this study, multiplex PCR panels were developed to amplify 20 microsatellite markers for red sea bream, , with the aim of reducing labor and material costs associated with genetic analysis of this species. The usefulness of these panels was validated in 200 fish collected at five sampling locations. The occurrence of null alleles was suggested at five markers, which were dropped from further analysis. The remaining 15 microsatellite loci showed high levels of heterozygosity ( (E) range 0.34-0.97, (O) range 0.32-1) and a wide range in the number of alleles per locus (; range 5-46). Increasing the number of microsatellite loci from three to ten and 15 improved the performance of the panels for population differentiation ( (ST)) and estimation of Nei's ( (S)) genetic distance. The results of this study confirm the usefulness of genotyping a large number of microsatellite DNA markers to expand our knowledge of red sea bream population genetics.