The high amino-acid content of sputum from cystic fibrosis patients promotes growth of auxotrophic Pseudomonas aeruginosa
The high amino-acid content of sputum from cystic fibrosis patients promotes growth of auxotrophic Pseudomonas aeruginosa
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DOI：
10.1099/00222615-45-2-110
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发表时间：
1996-08-01
影响因子：
3
通讯作者：
Pitt, TL
中科院分区：
文献类型：
--
作者：
Barth, AL;Pitt, TL
许多从囊性纤维化（CF）患者分离的铜绿假单胞菌是营养缺陷型的，需要氨基酸才能生长。采用定量分析法测定CF和非CF患者痰液溶胶相提取物中游离氨基酸的总含量，以评估气道中氨基酸的存在。定植有营养缺陷型铜绿假单胞菌的CF患者的痰液氨基酸含量（平均6.77 mg/ml）高于定植有原养菌的CF患者（平均3.77 mg/ml）;总体而言，CF标本（平均5.70 mg/ml）的氨基酸含量高于非CF样本（2.52 mg/ml）。通过一维薄层色谱法（TLC）评估痰提取物的氨基酸谱。在提取物中鉴定了几种氨基酸，特别是亮氨酸、异亮氨酸、苯丙氨酸、酪氨酸、丙氨酸、丝氨酸和甲硫氨酸或缬氨酸或两者。所有痰液标本，除两份（其中含有
Many isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients are auxotrophic and require amino acids for growth. A quantitative assay was used to determine the total content of free amino acids of sputum sol-phase extracts from CF and non-CF patients to assess the presence of amino acids in the airway. CF patients colonised with auxotrophic P. aeruginosa had a higher sputum amino-acid content (mean 6.77 mg/ml) than those colonised with prototrophs (mean 3.77 mg/ml); overall, CF specimens (mean 5.70 mg/ml) had a higher amino-acid content than non-CF samples (2.52 mg/ml). The amino-acid profile of sputum extracts was assessed by one-dimensional thin layer chromatography (TLC). Several amino acids were identified in the extracts, in particular, leucine, isoleucine, phenylalanine, tyrosine, alanine, serine and methionine or valine or both. All sputum specimens except two (which contained