Fluorescence Resonance Energy Transfer (FRET)
Fluorescence Resonance Energy Transfer (FRET)
复制标题
DOI：
10.1159/000209159
复制
发表时间：
2008-11
期刊：
影响因子：
--
通讯作者：
G. Vámosi;G. Vereb;A. Bodnár;K. Toth;Nina Baudendistel;S. Damjanovich
中科院分区：
文献类型：
--
作者：
G. Vámosi;G. Vereb;A. Bodnár;K. Toth;Nina Baudendistel;S. Damjanovich
FRET involves the excitation of a donor fluorophore by incident light within its absorption spectrum. This radiative absorption elevates the donor fluorophore to a higher-energy excited state that would normally decay (return to the ground state) radiatively with a characteristic emission spectrum. If, however, another fluorophore molecule (the acceptor) exists in proximity to the donor with its energy state characterized by an absorption spectrum that overlaps the emission spectrum of the donor, then the possibility of non-radiative energy transfer between donor and acceptor exists. As an example, Figure 1 shows the overlap of the cyan fluorescent protein (CFP) emission spectrum and the yellow fluorescent protein (YFP) absorption spectrum; this pair supports a strong FRET interaction.
