GENETIC-ANALYSIS OF MICROCIN H47 ANTIBIOTIC SYSTEM
GENETIC-ANALYSIS OF MICROCIN H47 ANTIBIOTIC SYSTEM
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DOI：
10.1128/jb.175.17.5420-5427.1993
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发表时间：
1993-09-01
影响因子：
3.2
通讯作者：
LAVINA, M
中科院分区：
文献类型：
--
作者：
GAGGERO, C;MORENO, F;LAVINA, M
微菌素H47基因决定簇跨越约10kb的DNA区域，代表了位于生产菌株染色体上的肠杆菌抗生素系统的第一次描述。与LacZ的转录和翻译融合显示了MicroCin H47系统的复杂转录组织。互补试验确定了生产抗生素所必需的六个基因；其中两个基因的产物参与了将微霉素输出到细胞外培养基中。免疫决定簇位于0.8kb的DNA片段中。在该系统中有一个大约3kb的推定的“沉默区域”，它可能与任何抗生素功能都不清楚地相关。蛋白质产物被鉴定并分配给三个生产基因和一个来自沉默区域的基因。
The microcin H47 genetic determinants span a DNA region of ca. 10 kb and represent the first description of an enterobacterial antibiotic system located in the chromosome of the producing strain. Transcriptional and translational fusions to lacZ showed a complex transcriptional organization of the microcin H47 system. Complementation tests identified six genes that are necessary for the production of the antibiotic; the products of two of them are involved in the export of microcin to the extracellular medium. The immunity determinant was located in an 0.8-kb DNA fragment. There is a putative ''silent region'' of ca. 3 kb inside the system that could not be clearly related to any antibiotic function. Protein products were identified and assigned to three production genes and also to a gene from the silent region.