CD4-T-cell antigen receptor complexes on human leukemia T cells.
CD4-T-cell antigen receptor complexes on human leukemia T cells.
复制标题
人类白血病 T 细胞上的 CD4-T 细胞抗原受体复合物。
DOI：
10.1073/pnas.87.13.5021
复制
发表时间：
1990
影响因子：
11.1
通讯作者：
Tse,DB
中科院分区：
文献类型：
--
作者：
Chuck,RS;Cantor,CR;Tse,DB
暴露于单克隆抗CD 4或抗TCR后，CD 4和T细胞抗原受体（TCR）从人和鼠T细胞表面共调节。这种共调节可能是因为CD 4和TCR的表达受类似的跨膜信号调节，或者因为CD 4和TCR在物理上相关联。为了研究质膜上的多分子组装，我们开发了一种流式细胞术方法，用于检测荧光素异硫氰酸酯（FITC）和四甲基罗丹明异硫氰酸酯（TRITC）-共轭的单克隆抗体作为敏化TRITC发射完整的单细胞之间的单线态-单线态能量转移。使用这个程序，我们检测到的转化的人白血病T细胞，HPB-ALL，在没有刺激的情况下，表面上的CD 4-TCR复合物。发现一种以上的CD 4与一种TCR相关。CD 4-TCR复合物不与游离CD 4和游离TCR快速平衡，并且它们不被染料标记的抗CD 4或抗TCR诱导。
CD4 and T-cell antigen receptor (TCR) comodulate from the surface of human and murine T cells following exposure to monoclonal anti-CD4 or anti-TCR. This comodulation may occur because expression of CD4 and TCR is regulated by similar transmembrane signals or because CD4 and TCR are physically associated. To study multimolecular assemblies on the plasma membrane, we developed a flow cytometric method for detecting singlet-singlet energy transfer between fluorescein isothiocyanate (FITC)- and tetramethylrhodamine isothiocyanate (TRITC)-conjugated monoclonal antibodies as sensitized TRITC emission on intact, single cells. Using this procedure, we detected CD4-TCR complexes on the surface of the transformed human leukemia T cells, HPB-ALL, in the absence of stimulation. More than one CD4 were found in association with one TCR. CD4-TCR complexes were not in rapid equilibrium with free CD4 and free TCR, and they were not induced by the dye-labeled anti-CD4 or anti-TCR.