Food-deprivation induces HSP70 and HSP90 protein expression in larval gilthead sea bream and rainbow trout
Food-deprivation induces HSP70 and HSP90 protein expression in larval gilthead sea bream and rainbow trout
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DOI：
10.1016/j.cbpb.2005.09.005
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发表时间：
2005-12-01
影响因子：
2.2
通讯作者：
Vijayan, MM
中科院分区：
文献类型：
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作者：
Cara, JB;Aluru, N;Vijayan, MM
热休克蛋白（HSPs）表达通常用作动物细胞应激的指标。然而，很少有人知道无论是热休克蛋白的表达模式或其作用的压力耐受现象在早期生命阶段的鱼类。为此，我们研究了食物剥夺（12小时），减少氧气水平（3.5毫克/升，1小时）和热休克（HS：+ 5摄氏度，1小时）对热休克蛋白70和热休克蛋白90的表达在早期生命阶段的金头鲷（Sparus aurata），一个温暖的水养殖物种。此外，我们调查了热休克蛋白70和热休克蛋白90的反应，在虹鳟鱼（虹鳟鱼mykiss），冷水养殖物种的早期生命阶段的食物剥夺（7天），和耐受性的这种幼虫热休克（无论是+5或+10摄氏度1小时）。我们的研究结果清楚地表明，食物剥夺增强两个物种的幼虫的HSP 70和HSP 90蛋白表达。在金头鲷仔鱼中，应激物诱导的HSP 70和HSP 90（仅在低氧组）蛋白表达在24 h恢复后恢复到未应激水平。在喂食的鳟鱼幼体中，+5 ℃的热休克没有提高HSP 70和HSP 90的表达，而+10 ℃的HS则明显导致100%的死亡率。然而，食物剥夺鳟鱼幼虫，其中有较高的HSP 70和HSP 90蛋白含量，生存HS和HS依赖性增加HSP 70，但不是HSP 90的表达。总体而言，HSP 70和HSP 90蛋白在早期生命阶段的鱼有可能被用作营养应激的标志物，而组织中的HSPs含量的升高可以被用作一种手段，以增加应激耐受性在幼虫饲养。(c)2005年爱思唯尔公司All rights reserved.
Heat shock proteins (HSPs) expression is commonly used as indicators of cellular stress in animals. However, very little is known about either the expression patterns of HSPs or their role in the stress-tolerance phenomenon in early life stages of fish. To this end, we examined the impact of food-deprivation (12 h), reduced oxygen levels (3.5 mg/L for 1 h) and heat shock (HS: + 5 degrees C for 1 h) on HSP70 and HSP90 protein expression in early life stages of the gilthead sea bream (Sparus aurata), a warm-water aquaculture species. Also, we investigated HSP70 and HSP90 response to food-deprivation (7 days) in early life stages of rainbow trout (Oncorhynchus mykiss), a cool-water aquaculture species, and the tolerance of this larvae to heat shock (either + 5 or + 10 degrees C for 1 h). Our results clearly demonstrate that food-deprivation enhances HSP70 and HSP90 protein expression in larvae of both species. In gilthead sea bream larvae, the stressors-induced HSP70 and HSP90 (only in the reduced oxygen group) protein expression returned to unstressed levels after 24 h recovery. In fed trout larvae, a +5 degrees C heat shock did not elevate HSP70 and HSP90 expression, whereas 100% mortality was evident with a + 10 degrees C HS. However, food-deprived trout larvae, which had higher HSP70 and HSP90 protein content, survived HS and showed HS-dependent increases in HSP70, but not HSP90 expression. Overall, HSP70 and HSP90 protein expression in early life stages of fish have the potential to be used as markers of nutritional stress, while elevation of the tissue HSPs content may be used as a means to increase stress tolerance during larval rearing. (c) 2005 Elsevier Inc. All rights reserved.