RENIN MESSENGER-RNA IS SYNTHESIZED LOCALLY IN RAT OCULAR-TISSUES
RENIN MESSENGER-RNA IS SYNTHESIZED LOCALLY IN RAT OCULAR-TISSUES
复制标题
DOI：
10.3109/02713689409047011
复制
发表时间：
1994-10-01
影响因子：
2
通讯作者：
WALLOW, IHL
中科院分区：
文献类型：
--
作者：
BRANDT, CR;PUMFERY, AM;WALLOW, IHL
肾素血管紧张素系统（RAS）的组成部分已在眼部组织和液体中检测到。眼部RAS蛋白的来源尚不清楚，但可能包括从体循环中扩散或渗漏、从血液中特异性摄取或局部合成。我们用RT-PCR和原位杂交（ISH）技术证实了3株大鼠眼部组织中存在肾素mRNA。通过RT-PCR，我们发现15个纤毛体样本中有10个，16个虹膜样本中有13个，3个视网膜样本中有1个肾素mRNA阳性。此外，6个脑部样本中有6个呈阳性，8个肾脏样本中有7个呈阳性。使用ISH，我们发现肾素mRNA在靠近巩膜的睫状肌延伸到脉络膜。前房角流出通道附近的组织也被标记。视网膜标记较弱，但存在于神经纤维层。用ISH在睫状体、虹膜和视网膜上也可以看到可能代表血管的颗粒团。这些结果提示存在局部眼部RAS。
Components of the Renin Angiotensin System (RAS) have been detected in ocular tissues and fluids. The source of the ocular RAS proteins is unknown but possibilities include diffusion or leakage from the systemic circulation, specific uptake from the blood, or local synthesis. We have used RT-PCR and in situ hybridization (ISH) to show that renin mRNA is present in ocular tissues from 3 strains of rats. By RT-PCR, we found 10 of 15 ciliary body samples, 13 of 16 iris samples, and 1 of 3 retina samples were positive for renin mRNA. Also, 6 of 6 brain and 7 of 8 kidney samples were positive. Using ISH, we found renin mRNA in the ciliary muscle adjacent to the sclera extending into the choroid. Tissue near the outflow channels of the anterior chamber angle also labeled. Retinal labeling was weak but present in the nerve fiber layer. Clusters of grains, possibly representing blood vessels, were also seen in the ciliary body, iris, and retina using ISH. These results suggest the presence of a local ocular RAS.