VALIDATING HIGH-THROUGHPUT MICRONUCLEUS ANALYSIS OF PERIPHERAL RETICULOCYTES FOR RADIATION BIODOSIMETRY: BENCHMARK AGAINST DICENTRIC AND CBMN ASSAYS IN A MOUSE MODEL
VALIDATING HIGH-THROUGHPUT MICRONUCLEUS ANALYSIS OF PERIPHERAL RETICULOCYTES FOR RADIATION BIODOSIMETRY: BENCHMARK AGAINST DICENTRIC AND CBMN ASSAYS IN A MOUSE MODEL
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DOI：
10.1097/hp.0b013e3181abaae5
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发表时间：
2010-02-01
期刊：
影响因子：
2.2
通讯作者：
Dertinger, Stephen D.
中科院分区：
文献类型：
--
作者：
Chen, Yuhchyau;Tsai, Ying;Dertinger, Stephen D.
辐射生物剂量测定的自动化是科学技术政策办公室和国土安全委员会考虑的首要任务之一，为国家做好准备应对可能的放射性核恐怖袭击。辐射后生物物理评估和风险管理中心是一个由研究人员和机构组成的联盟，以罗切斯特大学为中心，一直在研究对网织红细胞中辐射诱导的微核形成进行自动评分，以进行高通量辐射生物剂量测定。该合作项目基于纽约罗彻斯特 Litron 实验室的商用产品。该研究旨在通过与小鼠模型中基于标准淋巴细胞的生物剂量测定方法进行基准比较，验证用于辐射生物剂量测定的基于流式细胞术的微核网织红细胞表达分析。 C57B1/6 小鼠和 C3H 小鼠接受 0-3 Gy 的 Cs-137 全身辐射。随后通过流式细胞术分析血样的CD71+微核网织红细胞和网织红细胞频率。结果显示，C57B1/6 小鼠的 MN-RET 线性剂量反应高达 1 Gy，C3H 小鼠的 MN-RET 线性剂量反应高达 2 Gy。另一方面，通过基于淋巴细胞的双着丝粒测定和高达 3 Gy 的胞质分裂阻断微核测定获得了稳健且良好的剂量反应曲线。微核网织红细胞的高通量、自动化分析是检测近期辐射暴露的一种灵敏且可重复的方法。在小鼠中，检测剂量范围可高达 1 Gy (C57B1/6) 和 2 Gy (C3H)，但超出这些剂量限制就不可靠。目前正在研究将这种自动分析用于人体辐射生物剂量测定。健康物理。 98（2）：218-227； 2010年
Automation of radiation biodosimetry is one of the top priority tasks considered by the Office of Science and Technology Policy and the Homeland Security Council in preparation for the nation's readiness for a possible radionuclear terrorist attack. The Center for Biophysical Assessment and Risk Management Following Irradiation, a consortium of researchers and institutions centered at the University of Rochester, has been investigating automated scoring of radiation-induced micronucleus formation in reticulocytes for high-throughput radiation biodosimetry. The collaborative project is based on a commercially-available product by Litron Laboratories in Rochester, New York. The study was designed to validate the flow-cytometry based analysis of micronucleated reticulocyte expression for radiation biodosimetry by benchmarking against the standard lymphocyte-based biodosimetry methods in a mouse model. C57B1/6 mice and C3H mice were exposed to Cs-137 total-body radiation from 0-3 Gy. Blood samples were subsequently analyzed for CD71+ micronucleated reticulocyte and reticulocyte frequencies by flow cytometry. Results showed a linear dose-response of MN-RET up to 1 Gy for C57B1/6 and 2 Gy for C3H mice. On the other hand, robust and good dose-response curves were obtained with lymphocyte-based dicentric assay and cytokinesis-block micronucleus assay up to 3 Gy. High-throughput, automated analyses of micronucleated reticulocytes is a sensitive and reproducible method for detecting recent radiation exposure. In mice, the dose range of detection is useful up to 1 Gy (C57B1/6) and 2 Gy (C3H) but not reliable beyond these dose limits. The utilization of this automated analysis for human radiation biodosimetry is currently under investigation. Health Phys. 98(2):218-227; 2010