Systems analysis of biological networks in skeletal muscle function.
Systems analysis of biological networks in skeletal muscle function.
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骨骼肌功能生物网络的系统分析。
DOI：
10.1002/wsbm.1197
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Lieber,RichardL
中科院分区：
文献类型：
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作者：
Smith,LucasR;Meyer,Gretchen;Lieber,RichardL
骨骼肌的功能依赖于亚细胞系统之间的有效协调。这些系统由一组基因编码的蛋白质组成，所有这些基因都受到严格的调控。在由于疾病或受伤而改变调节的情况下，就会发生功能障碍。为了能够客观地分析肌肉基因表达谱，我们定义了9个生物网络，它们的协调对肌肉功能至关重要。我们开始通过描述最佳神经肌肉接头功能，导致肌肉细胞动作电位所必需的蛋白质的表达。这种动作电位被传递到参与兴奋-收缩偶联的蛋白质，从而使Ca 2+释放。然后，Ca 2+激活支持肌动蛋白和肌球蛋白跨桥循环的收缩蛋白。由交叉桥产生的力通过细胞骨架蛋白通过肌膜传递到支持肌肉细胞外基质的关键蛋白质。肌肉收缩是通过许多调节能量代谢的蛋白质来推动的。炎症是对损伤的常见反应，其可导致肌肉内许多途径的改变。肌肉也有多种途径，通过萎缩或肥大来调节大小。最后，与快肌纤维及其相应的亚型在慢肌纤维的亚型划定。这九个网络代表了影响骨骼肌功能的重要生物系统。将高通量系统分析与先进的网络软件相结合，将使研究人员能够使用这些网络来客观地研究骨骼肌系统.WIRES Syst Biol Med 2013，5：55-71.土井：10.1002/wsbm.1197这篇文章分类下：系统属性和过程模型>机械模型生理学>健康和疾病的哺乳动物生理学实验室方法和技术> RNA方法
Skeletal muscle function depends on the efficient coordination among subcellular systems. These systems are composed of proteins encoded by a subset of genes, all of which are tightly regulated. In the cases where regulation is altered because of disease or injury, dysfunction occurs. To enable objective analysis of muscle gene expression profiles, we have defined nine biological networks whose coordination is critical to muscle function. We begin by describing the expression of proteins necessary for optimal neuromuscular junction function that results in the muscle cell action potential. That action potential is transmitted to proteins involved in excitation–contraction coupling enabling Ca2+release. Ca2+then activates contractile proteins supporting actin and myosin cross‐bridge cycling. Force generated by cross‐bridges is transmitted via cytoskeletal proteins through the sarcolemma and out to critical proteins that support the muscle extracellular matrix. Muscle contraction is fueled through many proteins that regulate energy metabolism. Inflammation is a common response to injury that can result in alteration of many pathways within muscle. Muscle also has multiple pathways that regulate size through atrophy or hypertrophy. Finally, the isoforms associated with fast muscle fibers and their corresponding isoforms in slow muscle fibers are delineated. These nine networks represent important biological systems that affect skeletal muscle function. Combining high‐throughput systems analysis with advanced networking software will allow researchers to use these networks to objectively study skeletal muscle systems.WIREs Syst Biol Med2013, 5:55–71. doi: 10.1002/wsbm.1197This article is categorized under:Models of Systems Properties and Processes > Mechanistic ModelsPhysiology > Mammalian Physiology in Health and DiseaseLaboratory Methods and Technologies > RNA Methods