Using the TBAg/PHA ratio in the T-SPOT®. TB assay to distinguish TB disease from LTBI in an endemic area
Using the TBAg/PHA ratio in the T-SPOT®. TB assay to distinguish TB disease from LTBI in an endemic area
复制标题
使用 T-SPOT (R) 中的 TBAg/PHA 比率。
DOI：
10.5588/ijtld.15.0756
复制
发表时间：
2016-04-01
影响因子：
4
通讯作者：
Sun, Z-Y.
中科院分区：
文献类型：
--
作者：
Wang, F.;Hou, H-Y.;Sun, Z-Y.
SETTING: An important limitation of the T-SPOT (R) TB assay is its inability to distinguish active tuberculosis (TB) from latent tuberculous infection (LTBI).OBJECTIVE: We proposed a new calculation method for the T-SPOT assay and assessed its effect on distinguishing active TB from LTBI.DESIGN: A total of 162 active TB patients and 97 LTBI individuals were diagnosed according to conventional tests and the T-SPOT assay.RESULTS: The results of early secreted antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) in T-SPOT cannot be recommended for distinguishing TB from LTBI. The number of phytohaemagglutinin (PHA) spot-forming cells (sfc) in the T-SPOT assay was reduced in active TB patients. The ESAT-6/PHA or CFP-10/PHA ratios in active TB patients were significantly higher than in individuals with LTBI. Using 0.295 as the threshold ratio of Mycobacterium tuberculosis-specific antigen (TBAg) sfc to PHA sfc (TBAg/PHA ratio, the larger of ESAT-6/PHA and CFP-10/PHA), the sensitivity and specificity were 82.1% and 90.7% in distinguishing active TB from LTBI. The TBAg/PHA ratio might also be used to monitor the effect of anti-tuberculosis treatment.CONCLUSIONS: Calculating the TBAg/PHA ratio might have the potential to diagnose active TB and distinguish TB disease from LTBI.
