Indoxyl Sulfate Promotes Proliferation of Human Aortic Smooth Muscle Cells by Inducing Oxidative Stress
Indoxyl Sulfate Promotes Proliferation of Human Aortic Smooth Muscle Cells by Inducing Oxidative Stress
复制标题
DOI：
10.1053/j.jrn.2008.10.014
复制
发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Niwa, Toshimitsu
中科院分区：
文献类型：
--
作者：
Muteliefu, Gulinuer;Enomoto, Atsushi;Niwa, Toshimitsu
目的：心血管疾病是慢性肾病患者死亡的主要原因。我们最近证明，硫酸吲哚酚（IS）是一种尿毒症毒素，可诱导高血压大鼠的主动脉钙化和主动脉壁增厚。本研究旨在探讨IS是否促进人主动脉平滑肌细胞（HASMCs）增殖以及抗氧化剂是否抑制IS诱导的细胞增殖。方法：采用4-[3-(4-碘苯基)-2-(4-硝基苯基)-2H-5-四唑]-1,3-苯二磺酸盐检测50~500 μmol/L不同浓度IS对HASMCs细胞增殖的影响。 (WST-1) 测定。进一步测定了维生素E、维生素C、N-乙酰半胱氨酸等抗氧化剂对IS诱导的HASMCs增殖的影响。结果：IS显着促进HASMCs增殖，且呈浓度依赖性。抗氧化剂显着抑制IS诱导的HASMCs增殖。结论：IS通过诱导氧化应激促进HASMCs增殖。 (C) 2009 年，国家肾脏基金会，Inc. 保留所有权利。
Objectives: Cardiovascular disease is a major cause of mortality in chronic kidney disease patients. We have recently demonstrated that indoxyl sulfate (IS), a uremic toxin, induced aortic calcification and aortic wall thickening in hypertensive rats. This study aimed to determine if IS promotes proliferation of human aortic smooth muscle cells (HASMCs) and if antioxidants inhibit the IS-induced cell proliferation.Methods: We examined the effect of IS at different concentration from 50 to 500 mu mol/L on cell proliferation of HASMCs by using 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1) assay. Further, the effect of antioxidants such as vitamin E, vitamin C, and N-acetylcysteine on the IS-induced proliferation of HASMCs was determined.Results: IS significantly promoted the proliferation of HASMCs concentration-dependently. The antioxidants significantly inhibited the IS-induced proliferation of HASMCs.Conclusion: IS promotes proliferation of HASMCs by inducing oxidative stress. (C) 2009 by the National Kidney Foundation, Inc. All rights reserved.