Hyaluronic Acid-Decorated Laponite® Nanocomposites for Targeted Anticancer Drug Delivery
Hyaluronic Acid-Decorated Laponite® Nanocomposites for Targeted Anticancer Drug Delivery
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用于靶向抗癌药物输送的透明质酸修饰的 Laponite® 纳米复合材料
DOI：
10.3390/polym11010137
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发表时间：
2019-01-01
期刊：
影响因子：
5
通讯作者：
Guo, Rui
中科院分区：
文献类型：
--
作者：
Jiang, Tingting;Chen, Guangxiang;Guo, Rui
在这项研究中，透明质酸（HA），一种可以特异性结合CD 44受体的天然多糖，被缀合到laponite（R）（R）纳米盘上，用于抗癌药物阿霉素（DOX）的封装和特异性递送到CD 44过表达的癌细胞。所制备的LM-HA能够有效地包裹DOX，并具有pH响应性的持续释放药物。体外细胞活性实验证明LM-HA具有良好的生物相容性，载药LM-HA/DOX对CD 44受体过表达的HeLa细胞具有靶向抗肿瘤作用。流式细胞仪检测和激光共聚焦显微镜结果证实，LM-HA/DOX可通过CD 44介导的内吞作用被HeLa细胞特异性内化。因此，HA修饰的纳米盘具有高载药效率、pH敏感性和CD 44靶向性，可能是一种有效的肿瘤化疗纳米平台。
In this study, hyaluronic acid (HA), a natural polysaccharide that can specifically bind to CD44 receptors, was conjugated onto laponite((R)) (LAP) nanodisks for the encapsulation and specific delivery of the anti-cancer drug doxorubicin (DOX) to CD44-overexpressed cancer cells. The prepared LM-HA could encapsulate DOX efficiently and release drug in a continuous manner with pH-responsiveness. In vitro cell viability assay proved that LM-HA had good biocompatibility, and drug-loaded LM-HA/DOX exhibited targeted anti-tumor effects against HeLa cells with CD44 receptors overexpressed. In addition, the flow cytometric detection and confocal laser scanning microscope results confirmed that LM-HA/DOX could be specifically internalized by HeLa cells via CD44-mediated endocytosis. Therefore, the HA-modified LAP nanodisks with high drug loading efficiency, pH-sensitive drug release properties and CD44 targetability might be an efficient nanoplatform for cancer chemotherapy.