Dietary linoleic acid alters alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD), a key enzyme of niacin synthesis from tryptophan, in the process of protein expression in rat liver.
Dietary linoleic acid alters alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD), a key enzyme of niacin synthesis from tryptophan, in the process of protein expression in rat liver.
复制标题
膳食亚油酸会改变大鼠肝脏蛋白质表达过程中的α-氨基-β-羧基粘康酸-ε-半醛脱羧酶（ACMSD），这是色氨酸合成烟酸的关键酶。
DOI：
10.3177/jnsv.44.129
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发表时间：
1998
影响因子：
1.6
通讯作者：
H. Sanada
中科院分区：
文献类型：
--
作者：
Y. Egashira;A. Tanabe;T. Ohta;H. Sanada
α-氨基-β-羧基粘康酸-ε-半醛脱羧酶（ACMSD）[EC 4.1.1.45]是从色氨酸合成烟酸的关键酶。在这项研究中，我们研究了膳食亚油酸是否改变大鼠肝脏中ACMSD的蛋白质表达。用纯化的ACMSD酶免疫小鼠制备抗大鼠肝ACMSD抗体。使用这种多克隆抗体和双向电泳分析，我们研究了机制，其中肝脏ACMSD活性水平的变化，在大鼠喂养亚油酸饮食。在喂食亚油酸（L）的大鼠中，与喂食无脂饮食（FF）的大鼠相比，肝脏中的ACMSD蛋白水平受到强烈抑制。这些结果表明，ACMSD的表达水平可能受到亚油酸或其代谢产物的调节。
alpha-Amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD) [EC 4.1.1.45] is a key enzyme of niacin synthesis from tryptophan. In this study, we examined whether dietary linoleic acid alters the protein expression of ACMSD in rat liver. Antibody against rat liver ACMSD was prepared by injecting mice with the purified enzyme. With the use of this polyclonal antibody and analysis by two-dimensional electrophoresis, we studied the mechanism by which the level of liver ACMSD activity was varied in rats fed a linoleic acid diet. In the rats fed a dietary linoleic acid (L), ACMSD protein levels in the liver were strongly suppressed as compared with the rats fed a fat-free diet (FF). These results suggest that the expression level of ACMSD might be modulated by linoleic acid or their metabolites.