Highly Luminescent-Paramagnetic Nanophosphor Probes for In Vitro High-Contrast Imaging of Human Breast Cancer Cells
Highly Luminescent-Paramagnetic Nanophosphor Probes for In Vitro High-Contrast Imaging of Human Breast Cancer Cells
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DOI：
10.1002/smll.201200909
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发表时间：
2012-10-08
期刊：
影响因子：
13.3
通讯作者：
Ajayan, Pulickel M.
中科院分区：
文献类型：
--
作者：
Gupta, Bipin Kumar;Narayanan, Tharangattu N.;Ajayan, Pulickel M.
高发光顺磁性纳米荧光粉由于其在生物标记、生物成像和药物传递方面的潜在应用，在生物技术和生物医学研究中具有开创性的作用。本文报道了利用改进的溶胶技术合成高质量、超细、掺铕的氧化钇纳米荧光粉（y1.90 o3:Eu0.13+），并在人乳腺癌细胞中进行了体外荧光成像研究。这些平均粒径为6纳米的高发光纳米荧光粉提供了高对比度的光学成像和光散射的减少。荧光显微镜显示了体外细胞摄取情况，在246 nm紫外光激发下，Eu3+在610 nm （5D07F2）处具有特征性的强烈超敏红色发射峰。未观察到明显的细胞毒性。随后，时间分辨发射光谱和SQUID磁强计测量证明了以毫秒为单位的光致发光衰减时间和顺磁行为，这确保了纳米荧光粉在生物医学研究中的应用。
Highly luminescentparamagnetic nanophosphors have a seminal role in biotechnology and biomedical research due to their potential applications in biolabeling, bioimaging, and drug delivery. Herein, the synthesis of high-quality, ultrafine, europium-doped yttrium oxide nanophosphors (Y1.9O3:Eu0.13+) using a modified solgel technique is reported and in vitro fluorescence imaging studies are demonstrated in human breast cancer cells. These highly luminescent nanophosphors with an average particle size of 6 nm provide high-contrast optical imaging and decreased light scattering. In vitro cellular uptake is shown by fluorescence microscopy, which visualizes the characteristic intense hypersensitive red emission of Eu3+ peaking at 610 nm (5D07F2) upon 246 nm UV light excitation. No apparent cytotoxicity is observed. Subsequently, time-resolved emission spectroscopy and SQUID magnetometry measurements demonstrate a photoluminescence decay time in milliseconds and paramagnetic behavior, which assure applications of the nanophosphors in biomedical studies.