Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
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DOI：
10.1016/s0021-9258(18)43211-5
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发表时间：
1981-12
期刊：
影响因子：
--
通讯作者：
J. B. Olmsted
中科院分区：
文献类型：
--
作者：
J. B. Olmsted
本发明描述了一种使用已被电泳转移到重氮化纸上的蛋白质样品进行抗体亲和纯化的方法。使用分化的神经母细胞瘤细胞作为蛋白质样品和异质性抗微管蛋白血清，分离出仅特异性结合于印迹上的微管蛋白并且染色细胞中的微管网络的抗体。讨论了这种方法在各种类型应用中的一般实用性。
A method is described for the affinity purification of antibodies using protein samples that have been electrophoretically transferred to diazotized paper. Using differentiated neuroblastoma cells as the protein sample and a heterogeneous anti-microtubule protein serum, antibodies were isolated that specifically bound only to tubulin on blots and that stained microtubule networks in cells. The general utility of this method for various types of applications is discussed.