Guinea-pig alpha 1-fetoprotein: purification, characterization, developmental and hormonal regulation, and behavior in diethylnitrosamine hepatocarcinogenesis.
Guinea-pig alpha 1-fetoprotein: purification, characterization, developmental and hormonal regulation, and behavior in diethylnitrosamine hepatocarcinogenesis.
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豚鼠甲胎蛋白：纯化、表征、发育和激素调节以及二乙基亚硝胺肝癌发生中的行为。
DOI：
10.1139/o83-146
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
L. Bélanger
中科院分区：
文献类型：
--
作者：
H. Gourdeau;L. Bélanger
本文报道了纯化豚鼠甲胎蛋白（AFP）的理化和免疫学方法。建立了一种可检测0.1ng AFP的双抗体放射免疫分析法。豚鼠AFP的E1%278nm为6.6。其分子量为73000。其氨基酸组成与其他AFP相似，但由于甘露糖和N-乙酰葡糖胺的含量较高，因此其碳水化合物含量较高（8.5%）。由于其更基本的离子结构，豚鼠AFP具有比其他AFP更慢的电泳迁移率。它在非变性聚丙烯酰胺凝胶中分离成三种电泳变体;这些是等电点为5.0、5.12和5.54的电荷变体。神经氨酸酶处理后电泳迁移率的变化表明，电荷异质性是由于唾液酸的可变含量。豚鼠甲胎蛋白具有脂肪酸和凝集素结合特性，但对性激素没有亲和力。其生物半衰期为2.1天。它的合成部位是肝脏和卵黄囊，上消化道也有少量贡献。在妊娠第8周，肝脏和卵黄囊中AFP的产生似乎同步停止。从妊娠4至8周，AFP水平在胎儿血清中为2.5-3.5 mg/mL，在羊水中为0.1-0.2 mg/mL。电泳和凝集素反应性AFP变体的比例在发育过程中发生变化，反映了蛋白质糖基化的变化。妊娠期母体间甲胎蛋白水平表明通过羊膜交换达到了母胎平衡。正常成年豚鼠的血清AFP水平非常高，从400到2000 ng/mL，偶尔高达5000 ng/mL以上。地塞米松的施用抑制血清AFP水平。在二乙基亚硝胺诱发的肝癌发生过程中，血清AFP水平随着肿瘤的发展而适度升高。
Physicochemical and immunological procedures were developed for the purification of guinea-pig alpha 1-fetoprotein (AFP). A double-antibody radioimmunoassay was developed which can detect 0.1 ng of AFP. The E1%278 nm of guinea-pig AFP is 6.6. Its molecular weight is 73 000. Its amino acid composition is similar to other AFP's, but it has a higher carbohydrate content (8.5%), owing to larger amounts of mannose and N-acetylglucosamine. Guinea-pig AFP has a slower electrophoretic mobility than other AFP's, owing to its more basic ionic structure. It separates into three electrophoretic variants in nondenaturing polyacrylamide gels; these are charge variants with isoelectric points of 5.0, 5.12, and 5.54. Changes in electrophoretic mobility after neuraminidase treatment indicate that charge heterogeneity is due to a variable content in sialic acid. Guinea-pig AFP has fatty-acid- and lectin-binding properties, but no affinity for sex hormones. Its biological half-life is 2.1 days. Its sites of synthesis are the liver and the yolk sac, with minor contributions by the upper gastrointestinal tract. AFP production appears to cease synchronously in the liver and yolk sac during the 8th week of gestation. From 4 to 8 weeks of gestation, AFP levels are at 2.5-3.5 mg/mL in fetal serum and 0.1-0.2 mg/mL in amniotic fluid. The proportion of electrophoretic and lectin-reactive AFP variants changes during development, reflecting the changing glycosylation of the protein. The gestational levels of AFP in the maternal compartment are indicative of a fetomaternal equilibration through transamniotic exchange. Serum AFP levels in normal adult guinea pigs are remarkably high, from 400 to 2000 ng/mL and occasionally up to over 5000 ng/mL. The administration of dexamethasone suppresses serum AFP levels. During liver carcinogenesis induced by diethylnitrosamine, serum AFP levels increase, moderately, when tumors develop.