Doppler mode pulse sequences mitigate glomerular capillary hemorrhage in contrast-aided diagnostic ultrasound of rat kidney.
Doppler mode pulse sequences mitigate glomerular capillary hemorrhage in contrast-aided diagnostic ultrasound of rat kidney.
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多普勒模式脉冲序列减轻大鼠肾脏超声造影辅助诊断中的肾小球毛细血管出血。
DOI：
10.1109/tuffc.2007.464
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Wiggins,RogerC
中科院分区：
文献类型：
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作者：
Miller,DouglasL;Dou,Chunyan;Wiggins,RogerC
本研究旨在探讨诊断性超声造影剂破坏诱发大鼠肾小球毛细血管出血（GCH）的不同模式，以探讨可能的缓解策略。在水浴中以2.5 MHz扫描麻醉的无毛大鼠，通过尾静脉以10穆尔/kg/min输注造影剂。测试了B模式闪光回波成像（FEI）、彩色多普勒（CD）FEI和每秒1帧的实时多普勒成像，其图像脉冲序列的持续时间分别约为0.53 ms、15.8 ms和83.5 ms。生物效应终点包括大体观察到的充满血液的小管、GCH的组织学评价和血尿检测。B型FEI 1分钟，在2.6 MPa的峰值舒张压振幅（RPA）下，从扫描平面看，组织学上有38.6 ± 17.1%的肾小球出现GCH。GCH的阈值约为1.5 MPa，通过药剂输注暴露10分钟证实。CD模式FEI产生的脉冲数多而GCH数少（P < 0.02），实时多普勒模式FEI仅产生5.3 ± 3.8%（P < 0.005）。血尿结果遵循GCH趋势。这些发现表明了一种有前途的策略，即在试剂破坏图像脉冲序列中使用相对缓慢的脉冲RPA斜升，以减轻造影辅助诊断超声中的潜在生物效应。
Glomerular capillary hemorrhage (GCH) induced in rat kidney by diagnostic ultrasound involving contrast agent destruction was characterized for different modes to explore possible mitigation strategies. Anesthetized hairless rats were scanned at 2.5 MHz in a water bath with contrast agent infused at 10 mul/kg/minute via tail vein. B mode flash echo imaging (FEI), color Doppler (CD) FEI and realtime Doppler imaging at 1 frame per second were tested, which had image pulse sequences of approximately 0.53 ms, 15.8 ms, and 83.5 ms duration, respectively. Bioeffects endpoints included grossly observed blood-filled tubules, histological evaluation of GCH, and detection of hematuria. B mode FEI for 1 minute induced GCH in 38.6plusmn17.1% of glomeruli in histology from the scan plane for a peak rarefactional pressure amplitude (RPA) of 2.6 MPa. The threshold for GCH was approximately 1.5 MPa, confirmed by 10-minute exposure with agent infusion. Paradoxically, CD mode FEI delivered many more pulses but produced less GCH (P < 0.02), and real-time Doppler mode induced only 5.3plusmn3.8% (P < 0.005). Hematuria results followed the GCH trends. These findings indicate a promising strategy, which is to use relatively slow ramp-up of pulse RPAs in agent-destroying image pulse sequences, for mitigating potential bioeffects in contrast-aided diagnostic ultrasound.