Cytolytic T-cell granules: biochemical properties and functional specificity.
Cytolytic T-cell granules: biochemical properties and functional specificity.
复制标题
溶细胞 T 细胞颗粒：生化特性和功能特异性。
DOI：
10.1007/978-1-4684-8326-0_8
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发表时间：
1985
影响因子：
--
通讯作者：
Hengartner,H
中科院分区：
文献类型：
--
作者：
Podack,ER;Konigsberg,PJ;Acha-Orbea,H;Pircher,H;Hengartner,H
小鼠克隆的溶细胞NK和TK细胞可被归类为具有突出高尔基体复合体和活性肌浆体的大颗粒淋巴细胞（图1）。颗粒的特征在于由囊泡结构区域包围并由双层膜界定的致密均质核心。超微结构研究显示，颗粒定位在靶点-靶点结合物的结合位点，颗粒内容物明显释放（分泌）到间隙中（1-5）。随后，在靶膜和囊泡体上可检测到两种类型的膜损伤聚P1（直径160 μ m）和聚P2（直径约70 μ m）（图2）。这些研究导致了这样的假设，即颗粒含有负责膜损伤组装的因子，并且它们代表了克隆的溶细胞NK和TK细胞的溶细胞原理（7）。Henkart等人在抗体依赖性细胞毒性系统和表达NK样细胞毒性的大鼠淋巴瘤细胞系中得出了类似的结论。
Murine, cloned cytolytic NK- and TK-cells may be classified as large, granular lymphocytes possessing a prominent Golgi complex and active ergastoplasma (Fig. 1). The granules are characterized by a dense homogeneous core surrounded by an area of vesicular structures and delimited by a bilayer membrane. Ultrastructural studies showed the positioning of granules towards the conjugation site of killer-target conjugates and the apparent release (secretion) of granule contents into the interstitial space (1–5). Subsequently, two types of membrane lesions poly PI (160 Å diameter) and poly P2 (~70 Å diameter) became detectable on target membranes and on vescicular bodies (4,5,6) (Fig. 2). These studies led to the hypothesis that the granules contain the factors responsible for membrane lesion assembly and that they represent the cytolytic principle of cloned cytolytic NK and TK cells (7). Similar conclusions were reached by Henkart et al in the antibody dependent cytotoxicity system and with a rat lymphoma line that expressed NK-like cytotoxicity.