Characterization of Natural Antisense Transcript, Sclerotia Development and Secondary Metabolism by Strand-Specific RNA Sequencing of Aspergillus flavus
Characterization of Natural Antisense Transcript, Sclerotia Development and Secondary Metabolism by Strand-Specific RNA Sequencing of Aspergillus flavus
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通过黄曲霉链特异性 RNA 测序表征天然反义转录本、菌核发育和次生代谢
DOI：
10.1371/journal.pone.0097814
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发表时间：
2014-05
期刊：
影响因子：
3.7
通讯作者：
Wang, Bin
中科院分区：
文献类型：
--
作者：
Wu, Xinliang;Zhou, Bin;Yin, Chao;Guo, Yong;Lin, Ying;Pan, Li;Wang, Bin
黄曲霉因其对农业生产和由黄曲霉毒素引起的发酵产品的严重影响而受到广泛关注。菌核形态发生是黄曲霉繁殖和黄曲霉毒素合成的重要过程。为了获得黄曲霉广泛的转录组图谱并提供对这些生理过程的全面理解，我们对黄曲霉CA43培养物的mRNA进行了高通量链特异性RNA测序（ssRNA-seq）。我们的ssRNA-seq数据分析了黄曲霉基因组中广泛的转录，量化了大量转录本（占总基因的73%），并注释了精确的转录本结构，包括非翻译区、上游开放阅读框（orf）、可选剪接变异体和新转录本。我们认为黄曲霉天然反义转录物可能主要在转录后水平调控基因表达。这种调节可能与调节黄曲霉毒素生物合成和菌核发育等生物过程有关。菌丝体与菌核培养物差异表达基因的基因本体注释表明菌核发育与黄曲菌繁殖密切相关。此外，我们还建立了黄曲霉毒素生物合成的转录谱及其调控模型。我们发现了与菌核发育和黄曲霉毒素生物合成有关的潜在基因。这些基因可作为调控黄曲霉毒素菌株的靶点。
Aspergillus flavus has received much attention owing to its severe impact on agriculture and fermented products induced by aflatoxin. Sclerotia morphogenesis is an important process related to A. flavus reproduction and aflatoxin biosynthesis. In order to obtain an extensive transcriptome profile of A. flavus and provide a comprehensive understanding of these physiological processes, the isolated mRNA of A. flavus CA43 cultures was subjected to high-throughput strand-specific RNA sequencing (ssRNA-seq). Our ssRNA-seq data profiled widespread transcription across the A. flavus genome, quantified vast transcripts (73% of total genes) and annotated precise transcript structures, including untranslated regions, upstream open reading frames (ORFs), alternative splicing variants and novel transcripts. We propose natural antisense transcripts in A. flavus might regulate gene expression mainly on the post-transcriptional level. This regulation might be relevant to tune biological processes such as aflatoxin biosynthesis and sclerotia development. Gene Ontology annotation of differentially expressed genes between the mycelia and sclerotia cultures indicated sclerotia development was related closely to A. flavus reproduction. Additionally, we have established the transcriptional profile of aflatoxin biosynthesis and its regulation model. We identified potential genes linking sclerotia development and aflatoxin biosynthesis. These genes could be used as targets for controlled regulation of aflatoxigenic strains of A. flavus.
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影响因子：
4.4
作者：
Yavatkar AS;Lin Y;Ross J;Fann Y;Brody T;Odenwald WF
通讯作者：
Odenwald WF
影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
4.4
作者：
Sibthorp C;Wu H;Cowley G;Wong PW;Palaima P;Morozov IY;Weedall GD;Caddick MX
通讯作者：
Caddick MX
拟南芥顺式天然反义转录本的全基因组预测和鉴定。
影响因子：
12.3
作者：
Wang XJ;Gaasterland T;Chua NH
通讯作者：
Chua NH