Human γδ T Cells Augment Antigen Presentation in Listeria Monocytogenes Infection
Human γδ T Cells Augment Antigen Presentation in Listeria Monocytogenes Infection
复制标题
人类 γ δ T 细胞增强单核细胞增生李斯特菌感染中的抗原呈递
DOI：
10.2119/molmed.2015.00214
复制
发表时间：
2016-01-01
影响因子：
5.7
通讯作者：
He, Wei
中科院分区：
文献类型：
--
作者：
Zhu, Yuli;Wang, Huaishan;He, Wei
健康个体中的循环γ δ T细胞对细菌和病毒病原体迅速作出反应。许多研究表明，γ δ T细胞被单核细胞增生李斯特菌（李斯特菌）激活和扩增。单核细胞增多症），一种具有高致死率的食源性细菌病原体。然而，γ δ T细胞在L.单核细胞增多症感染尚不清楚。在本研究中，我们描述了L。单核细胞增多症感染，使用透射电子显微镜。结果显示，激活标志物包括人白细胞抗原DR（HLA-DR）和γ δ T细胞上的淋巴结归巢受体CCR 7在经L.单核细胞增多症外周血单个核细胞与经L.单核细胞增多症L.单核细胞增多症感染降低了小鼠上皮内淋巴细胞（IEL）中γ δ T细胞的百分比，并增加了体内γ δ T细胞表面上的MHC-II表达。我们的研究结果揭示了在L.单核细胞增生感染。
Circulating gamma delta T cells in healthy individuals rapidly respond to bacterial and viral pathogens. Many studies have demonstrated that gamma delta T cells are activated and expanded by Listeria monocytogenes (L. monocytogenes), a foodborne bacterial pathogen with high fatality rates. However, the roles of gamma delta T cells during L. monocytogenes infection are not clear. In the present study, we characterized the morphological characteristics of phagocytosis in gamma delta T cells after L. monocytogenes infection using transmission electron microscopy. Results show activation markers including human leucocyte antigen DR (HLA-DR) and lymph node-homing receptor CCR7 on gamma delta T cells were upregulated after stimulation via L. monocytogenes. Significant proliferation and differentiation of primary alpha beta T cells was also observed after coculture of peripheral blood mononuclear cells with gamma delta T cells anteriorly stimulated by L. monocytogenes. L. monocytogenes infection decreased the percentage of gamma delta T cells in mouse -intraepithelial lymphocytes (IELs) and increased MHC-II expression on the surface of gamma delta T cells in vivo. Our findings shed light on antigen presentation of gamma delta T cells during L. monocytogenes infection.