Patterning protein molecules on poly(ethylene glycol) coated Si(111).
Patterning protein molecules on poly(ethylene glycol) coated Si(111).
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DOI：
10.1016/j.biomaterials.2003.10.053
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发表时间：
2004-08
期刊：
影响因子：
14
通讯作者：
Yongseok Jun;Taewoon Cha;A. Guo;X. Zhu
中科院分区：
文献类型：
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作者：
Yongseok Jun;Taewoon Cha;A. Guo;X. Zhu
我们证明了高密度聚乙二醇（PEG）包覆的Si上的蛋白质分子的空间定位固定（111）。基于软光刻技术和醇官能团与端氯硅之间的有效反应，在硅表面形成了HO-和ch30端PEG区域的图案。ho端PEG刷的激活是通过部分氧化形成醛基团或通过有效的离去基团的附着来实现的。蛋白质分子被共价固定在PEG/Si表面的这些活化区域上。
We demonstrate spatially localized immobilization of protein molecules on high-density poly(ethylene glycol) (PEG) coated Si(111). Patterns of HO- and CH3O-terminated PEG regions are formed on silicon surfaces based on soft lithography techniques and an efficient reaction between alcohol functional groups and chlorine-terminated silicon. Activation of the HO-terminated PEG brush is achieved via either partial oxidation to form aldehyde groups or via attachment of efficient leaving groups. Protein molecules are covalently immobilized to these activated regions on the PEG/Si surface.