Moderate hypothermia increased the incidence of delayed paralysis through activation of the spinal microglia in an aortic cross-clamping rat model
Moderate hypothermia increased the incidence of delayed paralysis through activation of the spinal microglia in an aortic cross-clamping rat model
复制标题
在主动脉交叉钳夹大鼠模型中，中度低温通过激活脊髓小胶质细胞增加迟发性麻痹的发生率
DOI：
10.1016/j.ijcard.2016.06.169
复制
发表时间：
2016-10-01
影响因子：
3.5
通讯作者：
Dai, Ru-Ping
中科院分区：
文献类型：
--
作者：
He, Liang;Xu, Jun-Mei;Dai, Ru-Ping
背景：低温可减少主动脉瘤手术修复过程中的即刻瘫痪。然而，它是未知的低温是什么影响迟发性瘫痪，这种类型的surgery.Methods的严重并发症：Sprague-Dawley大鼠进行了闭塞的降主动脉在不同的持续时间下常温（38.0 +/- 0.5）或低温（33.0 +/- 0.5度）。评估神经功能。检测脊髓中运动神经元数量、胶质细胞活化和细胞因子表达。在主动脉阻断大鼠围手术期腹腔注射米诺环素。与当主动脉闭塞持续时间超过11.5分钟时发生立即瘫痪的常温条件相反，如果主动脉闭塞持续时间小于41分钟，则低温不会引起立即瘫痪。低温条件下，主动脉阻断时间超过18 min时，出现迟发性瘫痪，主动脉阻断时间为40 min时达到高峰。术后30 h运动神经元数目明显减少（P < 0.05）。此外，在低温组中，小胶质细胞被激活，并且白细胞介素-1 β和白细胞介素-6水平上调，这两者在术后24 h共定位于小胶质细胞中。二甲胺四环素治疗减弱的发病率和瘫痪的程度，但没有降低mortals.Conclusions：低温，在心胸外科手术的神经保护策略，通过激活脊髓小胶质细胞和细胞因子，延迟性瘫痪的发病率增加。阻断活化的小胶质细胞可能是预防迟发性瘫痪发生的潜在干预措施。(C)2016爱思唯尔爱尔兰有限公司版权所有。
Background: Hypothermia reduces immediate paralysis during surgical repair of aortic aneurysms. However, it is unknown what the impact of hypothermia is on delayed paralysis, a serious complication of this type of surgery.Methods: Sprague-Dawley rats were subjected to occlusion of the descending aorta at different duration under normothermia (38.0 +/- 0.5) or hypothermia (33.0 +/- 0.5 degrees). Neurologic function was assessed. Motor neuron number, glial activation, and cytokine expression in the spinal cord were examined. Minocycline was administered perioperatively by intraperitoneal injection in the rats subjected to the aorta occlusion.Results: In contrast to normothermia conditions at which immediate paralysis occurred when the duration of aorta occlusion exceeded 11.5 min, hypothermia did not induce immediate paralysis if the duration of aorta occlusion was less than 41 min. However, delayed paralysis was developed when the duration of aorta occlusion exceeded 18 min, and reached peak level when the duration of aorta occlusion was 40 min at hypothermia condition. The number of motoneurons was significantly decreased (P < 0.05) at 30 h postoperation. In addition, microglia was activated, and interleukin-1 beta and interleukin-6 levels were upregulated, both of which were co-localized in microglia at 24 h postoperation in the hypothermia group. Minocycline treatment attenuated the incidence and degree of paralysis but did not decrease the mortality.Conclusions: Hypothermia, a neuroprotective strategy in cardiothoracic surgery, increased the incidence of delayed paralysis through activation of spinal microglia and cytokines. Blocking the activated microglia may be a potential intervention to prevent the incidence of delayed paralysis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.