Distributions of p53 codon 72 polymorphism in bladder cancer - proline form is prominent in invasive tumor
Distributions of p53 codon 72 polymorphism in bladder cancer - proline form is prominent in invasive tumor
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DOI：
10.1007/s002400000117
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发表时间：
2000-10-01
影响因子：
--
通讯作者：
Li, CW
中科院分区：
文献类型：
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作者：
Chen, WC;Tsai, FJ;Li, CW
p53的异常功能通常与各种癌症形成相关。据报道，使用免疫组织化学染色时，高级别和晚期膀胱癌已发生突变或变为无活性p53。最近，p53密码子72多态性被广泛研究，以确定负责癌症形成的危险因素。野生型p53的密码子72序列多态性普遍存在。从G到C的单个碱基变化引起氨基酸残基72从精氨酸到脯氨酸的改变。精氨酸形式被认为是癌症发展的重要风险因素。然而.各种报告指出了关于这种多态性的差异;有些显示对照组和癌症组之间没有显著差异，而其他系列与脯氨酸形式纯合子的高风险有关。为了解决这种多态性在膀胱癌中的不确定分布，58名膀胱癌患者参加了本研究。当使用卡方检验（P = 0.952）进行检查时，对照受试者和膀胱癌患者之间的多态性分布没有差异。经Fisher精确检验，浸润性肝癌组脯氨酸型纯合子的检出率明显高于非浸润性肝癌组（分别为25%和2.9%，P < 0.001）。70%以上的非浸润性膀胱癌为精氨酸型纯合子。这一结果与Yu等报道的肝细胞癌中显示慢性肝病史和脯氨酸型纯合子的结果一致。我们的数据表明脯氨酸型纯合子与浸润性膀胱癌相关。
Abnormal function of p53 is commonly associated with various cancer formations. High-grade and late-stage bladder cancers have been reported to have mutated or become inactive p53 when using immunohistochemical stains. Recently, p53 codon 72 polymorphism was extensively studied to determine the risk factors responsible for cancer formation. There was a general population of codon 72 sequence polymorphism of the wild type p53. A single base change from G to C caused the alteration of amino acid residue 72 from arginine to proline. The arginine form is considered to be a significant risk factor in the development of cancer. However. various reports had indicated discrepancies with regard to this polymorphism; some showed no significant difference between the control and cancer groups, while other series were associated with high risks in the proline form homozygotes. To resolve the undefined distribution of this polymorphism in bladder cancers, 58 patients with bladder cancer were enrolled onto this study. When checked using the Chi-squared test (P = 0.952) there were no differences between the control subjects and bladder cancer patients in the distribution of polymorphism. However, proline form homozygotes were more frequently found in the invasive group than the non-invasive group by Fisher's exact test (25% and 2.9%, respectively, P < 0.001). More than 70% of the non-invasive bladder cancers were the arginine form homozygotes. This result is consistent with those reported for hepatocellular carcinoma that showed a history of chronic liver disease and proline form homozygotes in a report by Yu et al. Our data suggest that proline form homozygotes are associated with invasive bladder cancer.